| 应用DGGE法对青海相邻两盐湖中细菌多样性的快速检测 |
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| 引用本文： | 柴丽红,王涛,李沁元,崔晓龙,彭谦,徐丽华,姜成林.应用DGGE法对青海相邻两盐湖中细菌多样性的快速检测[J].生物学杂志,2003,20(1):13-14,19. |
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| 作者姓名： | 柴丽红 王涛 李沁元 崔晓龙 彭谦 徐丽华 姜成林 |
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| 作者单位： | 教育部微生物资源开放重点实验室,云南省微生物研究所,云南大学,昆明,650091 |
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| 基金项目： | 科技部基础研究重大项目前期研究专项 (项目编号 2 0 0 1CCC0 0 60 0 ) |
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| 摘 要： | 分别对青海相邻两盐湖柯柯盐湖、茶卡盐湖土样、泥样进行富集培养后，从中提取的DNA用两套不同的细菌通用引物进行扩增，分别得到包含V8和V9高变区的16SrDNA片断。经变性梯度凝胶电泳(DGGE)分析，结果显示这两个盐湖富集样品中细菌多样性具有较大的差异，而且同一盐湖不同性质样品中的细菌多样性差异也较大，两湖的泥样富集样品中均表现出了稍丰富的多样性。
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| 关 键 词： | DGGE法 青海 盐湖 细菌多样性 快速检测 土样 |
| 文章编号： | 1008-9632(2003)01-0013-02 |
Assessment of the bacterial community in the two adjacent lakes, Qinghai, by DGGE |
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| CHAI Li-hong,WANG Tao,LI Qin-yuan,CUI Xiao-long,PENG Qian,XU Li-hua,JIANG Cheng-lin.Assessment of the bacterial community in the two adjacent lakes, Qinghai, by DGGE[J].Journal of Biology,2003,20(1):13-14,19. |
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| Authors: | CHAI Li-hong WANG Tao LI Qin-yuan CUI Xiao-long PENG Qian XU Li-hua JIANG Cheng-lin |
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| Abstract: | Denaturing gradient gel electrophoresis (DGGE) was used to assess the enrichment bacterial community in two adjacent lakes,the Keke salt lake and the Chaka salt lake,Qinghai,China.DNA was extracted followed by amplification of 16S rDNA.Then PCR products which included the V8 and V9 high-variable region were analyzed by DGGE.The DGGE profiles showed that there were different bacterial communities in the two adjacent lakes and different bacterial communities existed in different samples of the same lake after enrichment,and the sludge samples showed more diversity both in the two lakes. |
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| Keywords: | DGGE Keke salt lake Chaka salt lake bacterial communities |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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