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| 道地药材霍山石斛及其相似种的分子鉴定 | |
| 引用本文： | 刘明珍,陈乃富,刘秀珍,邓辉,李耀亭,何祥林.道地药材霍山石斛及其相似种的分子鉴定[J].生物学杂志,2009,26(5):34-36. |
| 作者姓名： | 刘明珍 陈乃富 刘秀珍 邓辉 李耀亭 何祥林 |
| 作者单位： | 1. 皖西学院专科部,六安,237012 2. 植物细胞工程安徽省工程技术研究中心,六安237012;皖西学院化学与生命科学系,六安,237012 3. 巢湖学院体育系,巢湖,238000 4. 安徽省霍山县长冲中药材有限责任公司,霍山,237266 |
| 基金项目： | 皖西学院自然科学青年研究项目，植物细胞工程安徽省工程技术研究中心重点资助项目 |
| 摘 要： | 为检测霍山石斛与霍山产铜皮石斛、霍山产铁皮石斛及其它产地的几种药用石斛的差异，对霍山石斛及其相似种进行ISSR分子标记比较研究。结果表明，尽管霍山石斛及其相似种的外部形态差异较小，但在ISSR分子标记指纹图谱上却存在着显著而稳定的差异。因此，根据ISSR分子标记指纹图谱所显示的多态性位点差异，能准确鉴别霍山石斛及其相似种植物及药材。此方法简单、稳定性高、可重复性好，在霍山石斛的分子鉴定研究中有较大的应用价值。 |
| 关 键 词： | 霍山石斛 霍山产铜皮石斛 霍山产铁皮石斛 ISSR分子标记指纹图谱 鉴别 |
Molecular authentication of Dendrobium huoshanense from its allied species |
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| LIU Ming-zhen,CHEN Nai-fu,LIU Xiu-zhen,DENG Hui,LI Yao-ting,HE Xiang-lin.Molecular authentication of Dendrobium huoshanense from its allied species[J].Journal of Biology,2009,26(5):34-36. | |
| Authors: | LIU Ming-zhen CHEN Nai-fu LIU Xiu-zhen DENG Hui LI Yao-ting HE Xiang-lin |
| Institution: | LIU Ming-zhen CHEN Nai-fu LIU Xiu-zhen DENG Hui, LI Yao-ting, HE Xiang-lin ( 1. Specialist Deparment of West Anhui University, Liuan 237012; 2. Engineering and Technology Research Center of Plant Cell Engineering, Anhui Province. Liuan 237012 ; 3. Chemistry and Life Science Deparment of West Anhui University,. Liuan 237012; 4. Physical Education Deparment of Chao Hu University, Chaohu 238000; 5. Changchong Traditional Chinese Medicinal Materials Company of Huoshan in Anhui, Huoshan 237266, China) |
| Abstract: | To distinguish Dendrobium huoshanense from its allied species like D. officinale, D. moniliforme and D. hercoglossum, ISSR fingerprint was used to compare their diversity. Although the morphological difference was slight, molecular difference among them determined by ISSR fingerprint was obvious and stable. Results suggested that the difference of ISSR fingerprint can be used to authenticate accurately D. huoshanense from its allied species. Method of fingerprint of ISSR marker for Dendrobium huoshanense classification is simple, stable and repeatable. It will be helpful for molecular authentication study on Dendrobium huoshanense. |
| Keywords: | D huoshanense D officinale D moniliforme ISSR fingerprint authentication |
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