| 诱导茶树成熟胚培育成幼苗的研究 |
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| 引用本文： | 蔡海云,江昌俊,王朝霞,宛晓春.诱导茶树成熟胚培育成幼苗的研究[J].生物学杂志,2007,24(2):21-23. |
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| 作者姓名： | 蔡海云 江昌俊 王朝霞 宛晓春 |
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| 作者单位： | 安徽农业大学茶叶生物化学与生物技术国家重点实验室,安徽,合肥,230036 |
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| 摘 要： | 以茶树品种——农抗早的成熟胚为外植体离体培养,初次研究了2,4-D、6-BA等不同植物激素对茶树成熟胚诱导成幼苗的影响。结果表明,在合适的激素组合条件下,愈伤组织诱导为97%,在附加2.5mg/L 6-BA 0.5mg/L NAA的MS培养基上,愈伤组织的芽分化率为14.2%,附加1.5mg/L NAA的1/2 MS培养基,幼苗的根分化率为5%。
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| 关 键 词： | 茶树 成熟胚 愈伤组织 离体培养 |
| 文章编号： | 1008-9632（2007）02-0021-03 |
| 修稿时间： | 2006-04-13 |
Study on the induced plantlet from mature embryo of tea plant |
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| CAI Hai-yun,JIANG Chang-jun,WANG Zhao-xia,WAN Xiao-chun.Study on the induced plantlet from mature embryo of tea plant[J].Journal of Biology,2007,24(2):21-23. |
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| Authors: | CAI Hai-yun JIANG Chang-jun WANG Zhao-xia WAN Xiao-chun |
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| Abstract: | Mature embryos from cultivar Nong Kang Zao were used as explants to firstly study the effect of plants growth with different plant growth regulators like 2,4-D and 6-BA.The results showed that with suitable plant growth regulators induction rate of callus was 97%.Shoot differentiation medium was MS medium supplemented with 6-BA 2.5 mg/L and NAA 0.5 mg/L,which resulted in 14.2% shoot regeneration frequency,the 1/2 MS medium with 1.5 mg/L NAA was suitable for young plant growth,which resulted in 5% root formation frequency. |
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| Keywords: | tea plant mature embryo callus tissue in vitro culture |
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