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| 分子标记辅助选择聚合Xa23,Pi9和Bt基因 | |
| 引用本文： | 陈圣,倪大虎,陆徐忠,李莉,王文相,章琦,赵开军,张亮,郭霞,杨剑波.分子标记辅助选择聚合Xa23,Pi9和Bt基因[J].生物学杂志,2009,26(3):7-9. |
| 作者姓名： | 陈圣 倪大虎 陆徐忠 李莉 王文相 章琦 赵开军 张亮 郭霞 杨剑波 |
| 作者单位： | 1. 安徽大学生命科学学院,合肥,230039;安徽省农业科学院水稻研究所,合肥,230031 2. 安徽省农业科学院水稻研究所,合肥,230031 3. 安徽省农业科学院植物保护研究所,合肥,230031 4. 中国农业科学院作物科学研究所,北京,100081 |
| 基金项目： | 国家科技支撑计划子课题 |
| 摘 要： | 利用分子标记辅助选择将高抗水稻白叶枯病的Xa23基因、广谱高抗稻瘟病的Pi9基因、抗水稻螟虫和稻纵卷叶螟的Bt基因聚合到同一株系中,获得了三基因聚合的纯合株系。病、虫抗性鉴定结果显示：聚合了Xa23、Pi9和Bt基因的株系HB1471、HB1473能同时抗白叶枯病、稻瘟病和稻纵卷叶螟;与Xa23、Pi9基因的供体材料L10相比,对白叶枯病和稻瘟病的抗谱相同、抗性水平相当;对稻纵卷叶螟抗性与Bt基因的供体亲本MH63-Bt水平相当。Xa23、Pi9和Bt三基因纯合株系可以作为水稻育种的多抗供体材料。 |
| 关 键 词： | 分子标记辅助选择聚合Xa23 Pi9和Bt基因 |
Pyramiding Xa23, Pi9 and Bt genes by molecular marker-assisted selection |
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| CHEN Sheng,NI Da-hu,LU Xu-zhong,LI Li,WANG Wen-xiang,ZHANG Qi,ZHAO Kai-jun,ZHANG Liang,GUO Xia,YANG Jian-bo.Pyramiding Xa23, Pi9 and Bt genes by molecular marker-assisted selection[J].Journal of Biology,2009,26(3):7-9. | |
| Authors: | CHEN Sheng NI Da-hu LU Xu-zhong LI Li WANG Wen-xiang ZHANG Qi ZHAO Kai-jun ZHANG Liang GUO Xia YANG Jian-bo |
| Institution: | CHEN Sheng,NI Da-hu,LU Xu-zhong,LI Li,WANG Wen-xiang,ZHANG Qi,ZHAO Kai-jun,ZHANG Liang,GUO Xia, YANG Jian-bo(1. School of Life Science, Anhui University, Hefei 230039 ;2. Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031 ;3. Plant Protection Institute, Anhui Academy of Agricultural Sciences, Hefei 230031 ;4. Crop Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China) |
| Abstract: | Xa23, Pi9 and Bt genes which possess wide resistance spectrum and high resistance level respectively to bacterial blight（ BB）, fungi blast, stemborers and leaffolders of rice were pyramided by molecular marker-assisted selection（MAS）. Two homozygous lines HB1471 and HB1473 carrying the three resistant genes were obtained. Resistance identification showed that the pyramided lines HB1471 and HB1473 simultaneously possessed high resistance level to BB, blast and leaffolders （Cnaphalocrocis medialis）. Compared with the Xa23 and Pi9 genes donor parent L10, the pyramided lines had the similar resistance spectrum and level to BB and blast. Meanwhile, they exhibited equivalent resistance to leaffolders with Bt donor parent MH63-Bt. The homozy- gous lines carrying Xa23, Pi9 and Bt genes should be very useful in rice breeding as donor of multiple resistance genes. |
| Keywords: | Xa23 Pi9 Bt genes pyramiding molecular marker-assisted selection |
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