| 增强UV-B辐射对小麦叶肉细胞原生质体微丝骨架的影响 |
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| 引用本文： | 翟菁如,陈慧泽,高丽美,李永峰,韩榕.增强UV-B辐射对小麦叶肉细胞原生质体微丝骨架的影响[J].生物学杂志,2010,27(6):33-36. |
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| 作者姓名： | 翟菁如 陈慧泽 高丽美 李永峰 韩榕 |
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| 作者单位： | 山西师范大学,生命科学学院,临汾,041004 |
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| 基金项目： | 国家自然科学基金资助项目，山西省自然科学基金资助项目 |
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| 摘 要： | 以增强UV-B（10.08 kJ/m2.d）辐射后的小麦幼苗叶肉细胞原生质体为材料,异硫氰酸荧光素标记的鬼笔环肽（FITC-Ph）为探针,利用激光共聚焦扫描显微镜,观察分析小麦叶肉细胞原生质体中微丝骨架的分布及形态变化。结果表明对照组中,叶肉细胞原生质体微丝呈现网状或平行状随机排列,形态上表现为纤维状结构。增强UV-B辐射处理后,原生质体中微丝的密集分布遭到破坏,纤丝状微丝消失,聚集成束,或呈点状、碎片状分布。
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| 关 键 词： | 增强UV-B 小麦 原生质体 微丝 激光共聚焦扫描显微镜 |
Influence of enhanced UV-B radiation on microfilament cytoskeleton in wheat mesophyll protoplast |
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| ZHAI Jing-ru,CHEN Hui-ze,GAO Li-mei,LI Yong-feng,HAN Rong.Influence of enhanced UV-B radiation on microfilament cytoskeleton in wheat mesophyll protoplast[J].Journal of Biology,2010,27(6):33-36. |
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| Authors: | ZHAI Jing-ru CHEN Hui-ze GAO Li-mei LI Yong-feng HAN Rong |
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| Institution: | (College of Life Science,Shanxi Normal University,Linfen 041004,China) |
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| Abstract: | Microfilaments（MFs） were observed by confocal laser scanning microscopy（CLSM） in the wheat mesophyll protoplast treated with FITC-Phalloidin.Two types of MFs bundles could be recognized.They were beneath the plasma membrane parallel to each other surrounding the chloroplast.When the wheat cells were treated by enhanced ultraviolet-B radiation,the distribution pattern of MFs was changed form the form of net to bundles or granules. |
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| Keywords: | enhanced UV-B radiation wheat protoplast microfilament（MF） confocal laser scanning microscope（CLSM） |
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