| 太和香椿总黄酮的提取及含量测定 |
| |
| 引用本文： | 杨阳,杨京霞,王荣,李文雍.太和香椿总黄酮的提取及含量测定[J].生物学杂志,2011,28(1):91-93. |
| |
| 作者姓名： | 杨阳 杨京霞 王荣 李文雍 |
| |
| 作者单位： | 1. 安徽大学生命科学学院,合肥,230039
2. 阜阳师范学院生命科学学院,阜阳,236041;抗衰老中草药安徽省工程技术研究中心,阜阳,236041 |
| |
| 基金项目： | 安徽高校省级自然科学研究规划产学研重点项目,安徽省教育厅自然科学研究项目 |
| |
| 摘 要： | 设计正交实验对酶解水提法进行优化,以浸提液中总黄酮得率作为考察指标。采用最佳提取方法提取太和产香椿5个品种的总黄酮,比较其含量大小。结果表明酶解水提法的最佳提取条件是料液比1∶40。酶用量15U/g,酶解时间90min,酶解后水提时间120min。红油椿的总黄酮含量最高,黑油椿次之,其次是红椿、青椿,毛椿的总黄酮含量最少。
|
| 关 键 词： | 香椿 太和县 总黄酮 纤维素酶 |
The extraction and determination of total flavonoids of Toona sinesis cultivated in Taihe county |
| |
| YANG Yang,YANG Jin-xia,WANG Rong,LI Wen-yong.The extraction and determination of total flavonoids of Toona sinesis cultivated in Taihe county[J].Journal of Biology,2011,28(1):91-93. |
| |
| Authors: | YANG Yang YANG Jin-xia WANG Rong LI Wen-yong |
| |
| Institution: | YANG Yang1,YANG Jin-xia2.3,WANG Rong2,LI Wen-yong2,3(1.College of Life Science,Anhui University,Hefei 230039,2.College of Life Science,Fuyang Normal University,3.Engineering Technology Research Center of Anti-aging Chinese Herbal Medicine,Fuyang 236041,China) |
| |
| Abstract: | To optimize the extraction process of total flavonoids of Toona sinensis and to compare its total flavonoids among five different species cultivated in Taihe county,Anhui province,China,the orthogonal experiment was designed for optimizing enzyme treatment by referring the yield of total flavonoids in extraction.Result showed that the best extraction condition was as follow： the ratio between solid to liquid 1∶40,enzyme activity 15U/g,the hydrolysis time 90min and the extraction time after hydrolysis 120min.The total flavonoid content of red oil toona was the highest,followed by black oil toona,red toona,green toona,and hairy toona is the least. |
| |
| Keywords: | Toona sinensis Taihe county total flavonoids cellulase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
