| 石蒜组织培养和植株再生的研究 |
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| 引用本文： | 朱景存,张玉琼,刘春滟,马毛迎.石蒜组织培养和植株再生的研究[J].生物学杂志,2010,27(6):46-48. |
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| 作者姓名： | 朱景存 张玉琼 刘春滟 马毛迎 |
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| 作者单位： | 安徽农业大学生命科学学院,合肥,230036 |
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| 基金项目： | 国家自然科学基金资助项目30570189 |
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| 摘 要： | 以石蒜的双鳞片为外植体,MS为培养基,对石蒜组织培养适宜条件进行筛选,并对石蒜的植株再生进行了研究。结果表明：MS＋3mg/L6-BA＋0.3mg/L NAA是不定芽增殖的最佳激素组合,诱导率可达73.99%;石蒜生长旺盛期的11月是芽诱导率最高的时期,同时,MS＋0.5mg/L6-BA＋0.8mg/L NAA是较好的生根培养基,生根率可达95.00%;幼苗移栽至蛭石30%营养土中移栽成活率最高达91.67%。
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| 关 键 词： | 石蒜 双鳞片 植株再生 |
Study on tissue culture and the plantlet regeneration of Lycoris radiata(L'Her.) |
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| ZHU Jing-cun,ZHANG Yu-qiong,LIU Chun-yan,MA Mao-ying.Study on tissue culture and the plantlet regeneration of Lycoris radiata(L'Her.)[J].Journal of Biology,2010,27(6):46-48. |
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| Authors: | ZHU Jing-cun ZHANG Yu-qiong LIU Chun-yan MA Mao-ying |
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| Institution: | (School of Life Science,Anhui Agricultural University,Hefei 230036,China) |
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| Abstract: | Effects of phytohormones in different concentrations on the tissue culture and plant regeneration were investigated with the twin-scaling of the Lycoris radiata（L′Her.） as explants.The results showed that the optimal mediums for the adventitious buds induction was MS＋3mg/L6-BA ＋0.3mg/L NAA with the induction rate 73.99%.Rooting rate on the optimal rooting medium was 95.00%.As the vigorous growth period of the Lycoris radiata,November was the best period of buds induction.The transplanting survival rate of plantlets was 91.67% in the suitable proportion of transplanting medium（the soil ＋30%vermiculite）. |
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| Keywords: | Lycoris radiata twin-scaling the plant regeneration |
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