转双价基因抗虫棉及其杂交F1代对棉铃虫幼虫的抗性研究

利用室内生物测定法和田间调查法研究双价抗虫棉及其杂交F1代不同生育期主茎功能叶和花铃期不同组织器官的抗虫性。结果表明:双价抗虫棉及其杂交F1代的抗虫性变化规律与B t基因棉的抗虫性变化规律相近,为生育前期(苗期、蕾期)抗性强,生育后期(花铃期、吐絮期)抗性弱,在花铃期为:棉铃>棉蕾>花>棉叶,且差异极显著。与B t基因棉相比,生育前期双价抗虫棉及其杂交F1代的抗虫性略弱,但差异不显著;生育后期双价抗虫棉及其杂交F1代的抗虫性显著强于B t基因棉。

Study on the resistances of the F1 hybrids and the transgenic Bt+CpTI cotton lines to Helicoverpa armigera

Laboratory bioassay and field experiment were conducted to study the resistances of leaves from the main stem at deveopment stages and different cotton organs at flowering-boll stage to Helicoverpa arrnigera.Results showed as follows: Laboratory bioassay determine indicated a similar mode of change in double-copy transgenic cotton,F_1 hybrids and transgenic Bt gene cotton that resistance was higher at earlier development stages while declined at later stages.The resistance at flowering-boll stage was: boll>squre>flower>mature leaf on main stem.The leaves,buds,flowers and bolls of double-copy transgenic cotton and F_1 hybrids during later developmental stage(flowering-boll stage)were not damaged in the experimental arer and presented significant resistance to cotton bollworms.