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| 重组人瘦素蛋白纯化鉴定及其单克隆抗体制备 | |
| 引用本文： | 谢小强,曹亮,王弦,徐振山,宋礼华.重组人瘦素蛋白纯化鉴定及其单克隆抗体制备[J].生物学杂志,2009,26(3):26-28. |
| 作者姓名： | 谢小强 曹亮 王弦 徐振山 宋礼华 |
| 作者单位： | 1. 安徽医科大学生物化学与分子生物学教研室,合肥,230032 2. 安徽大学生命科学学院,合肥,230039 3. 安徽安科生物工程(集团)股份有限公司,合肥,230088 4. 安徽医科大学生物化学与分子生物学教研室,合肥,230032;安徽安科生物工程(集团)股份有限公司,合肥,230088 |
| 摘 要： | 大肠杆菌（E.coli）重组表达获得的重组人瘦素蛋白（rh-leptin）,复性、纯化后进行SDS-PAGE电泳和Western-blot印迹杂交鉴定其免疫学活性,免疫小鼠后制备单克隆抗体,结果表明通过对rh-leptin进行复性和纯化,获得了高纯度的具有免疫学活性的rh-leptin蛋白,并获得一株稳定分泌抗rh-leptin单抗的杂交瘤细胞株。瘦素蛋白的纯化及其单克隆抗体的制备,可供瘦素进一步研究应用。 |
| 关 键 词： | 瘦素 蛋白纯化 单克隆抗体 离子交换层析 |
Purification and identification of rh-leptin and preparation of rh-leptin monoclonal antibody |
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| XIE Xiao-qiang,CAO Liang,WANG Xian,XU Zhen-shan,SONG Li-hua.Purification and identification of rh-leptin and preparation of rh-leptin monoclonal antibody[J].Journal of Biology,2009,26(3):26-28. | |
| Authors: | XIE Xiao-qiang CAO Liang WANG Xian XU Zhen-shan SONG Li-hua |
| Institution: | XIE Xiao-qiang, CAO Liang, WANG Xian, XU Zhen-shan,SONG Li-hua( 1. Department of Biochemistry and Molecule Biology, Anhui Medical University, Hefei, 230032; College of Life Science, Anhui University, Hefei 230039 ; 3. Anhui Anke Bioteehnology (Group) Co. ,Ltd, Hefei 230088,China ) |
| Abstract: | The immunological activity of recombined human leptin （rh-leptin） after the protein renaturation and purification was identified by SDS-PAGE and immune mouse with rh-leptin to preparation monoelonal antibody for preparation monoelonal anti- body of reeombined human leptin and purification of reeombined human rh-leptin. Results showed that highly purified rh-leptin with immunological activity by rh-leptin protein renaturation and purification was obtained, and one strain of hybridoma cell can stably secrete MCAB of rh-leptin. By this method, preparation monoelonal antibody of reeombined human leptin and purification of recombined human leptin may be used in further research. |
| Keywords: | rh-leptin protein purification monoclonal antibody ion exchange chromatography |
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