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| 利用DGGE法研究不同种植体系中根际微生物群落结构 | |
| 引用本文： | 宋亚娜,包兴国,李隆,张福锁,郑伟文.利用DGGE法研究不同种植体系中根际微生物群落结构[J].生物学杂志,2006,23(5):12-15. |
| 作者姓名： | 宋亚娜 包兴国 李隆 张福锁 郑伟文 |
| 作者单位： | 1. 中国农业大学资源环境学院,北京,100094;福建农科院生物技术研究所,福州,350003 2. 甘肃农科院土壤肥料研究所,兰州,730000 3. 中国农业大学资源环境学院,北京,100094 4. 福建农科院生物技术研究所,福州,350003 |
| 基金项目： | 农业部资助948项目(项目编号2003-Z53) |
| 摘 要： | 利用DGGE技术研究不同间作和轮作种植体系对作物根际细菌和真菌群落结构的影响.运用16SrDNA和18SrDNA特异引物对,将土壤中提取的总DNA进行PCR扩增后,通过DGGE技术对PCR产物进行分析,结果表明:玉米-蚕豆轮作对蚕豆根际细菌和真菌群落结构影响明显,二者都与单作蚕豆有较大差异;小麦/蚕豆间作明显改变两种作物根际细菌群落结构和蚕豆根际真菌群落结构;玉米/蚕豆间作明显改变玉米根际细菌、真菌群落结构和蚕豆根际真菌群落结构. |
| 关 键 词： | DGGE 细菌群落结构 真菌群落结构 间作 轮作 |
| 文章编号： | 1008-9632(2006)05-0012-04 |
| 修稿时间： | 2006年3月27日 |
Study of rhizosphere microbial community composition in different cropping systems by DGGE |
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| SONG Ya-na,BAO Xing-guo,LI Long,ZHANG Fu-suo,ZHEN Wei-wen.Study of rhizosphere microbial community composition in different cropping systems by DGGE[J].Journal of Biology,2006,23(5):12-15. | |
| Authors: | SONG Ya-na BAO Xing-guo LI Long ZHANG Fu-suo ZHEN Wei-wen |
| Abstract: | Rhizosphere bacterial and fungal communities composition were studied by denaturing gradient gel electrophoresis(DGGE) of 16S rDNA and 18S rDNA in different cropping systems.The results showed that rotation of maize and faba bean changed the bacterial and fungal communities composition in rhizosphere of faba bean compared to sole cropping.Intercropping of wheat and faba bean altered the bacterial community composition in rhizosphere of wheat and faba bean and the fungal community composition in rhizosphere of faba bean.And intercropping of maize and faba bean shifted the bacterial and fungal communities composition in rhizosphere of maize and the fungal community composition in rhizosphere of faba bean compared to sole cropping. |
| Keywords: | DGGE bacterial community fungal community intercropping rotation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |