| 转基因枸杞中蛋白酶体糜蛋白酶样活性的研究 |
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| 引用本文： | 刘宁,宋长征.转基因枸杞中蛋白酶体糜蛋白酶样活性的研究[J].生物学杂志,2009,26(6):1-4. |
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| 作者姓名： | 刘宁 宋长征 |
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| 作者单位： | 卫生部生物技术药物重点实验室,山东省医药生物技术研究中心,山东省医学科学院,济南,250062 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 利用提取的转基因枸杞和正常枸杞蛋白与蛋白酶体的特异性荧光底物Suc-LLVY-AMC室温孵育,于360nm（激发光）/460nm（发射光）波长下测定荧光值的方法,研究了转基因枸杞和正常枸杞中蛋白酶体糜蛋白酶活性的差异及其特异性抑制剂MG115对枸杞中蛋白酶体活性的影响。结果表明表达H IV壳体蛋白的转基因枸杞蛋白酶体活性是空载体对照转基因枸杞蛋白酶体活性的3.6倍,是正常枸杞的4.2倍。10μM的MG115对表达H IV壳体蛋白的转基因枸杞蛋白酶体活性抑制率为87%,携带空载体对照的转基因枸杞抑制率为74%,而对正常枸杞抑制率仅为8.6%;50μM的MG115抑制作用与10μM的相比没有明显变化。这一结果将为利用蛋白酶体抑制剂提高转基因枸杞悬浮细胞H IV-1CA含量的研究提供理论依据。
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| 关 键 词： | 转基因枸杞 人类免疫缺陷病毒 壳体蛋白 蛋白酶体 |
Research on chymotryptic activity of the 20S proteasome in transgenic Lycium barbarum L. |
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| LIU Ning,SONG Chang-zheng.Research on chymotryptic activity of the 20S proteasome in transgenic Lycium barbarum L.[J].Journal of Biology,2009,26(6):1-4. |
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| Authors: | LIU Ning SONG Chang-zheng |
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| Institution: | LIU Ning,SONG Chang-zheng(Key Laboratory for Biotech-Drugs of Ministry of Health,Shandong Medicinal Biotechnology Center,Shandong Academy of Medical Science,Jinan 250062,China) |
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| Abstract: | The difference of chymotrypsin-like activity in proteasome between transgenic Lycium barbarum and non-transgenic Lycium barbarum was investigated.The change of the proteasome activity in the presence or absence specific proteasome inhibitor MG115 was also observed.The proteins of transgenic Lycium barbarum and non-transgenic Lycium barbarum were isolated and incubated with fluorescent substrate Suc-LLVY-AMC specific for chymotrypsin-like activity.The changes of fluorescence intensity were measured at 360 nm... |
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| Keywords: | Transgenie Lycium barbarum human immunodeficiency virus(HIV) capside proteasome |
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