| 黑斑蛙骨髓细胞染色体标本制备的新方法 |
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| 引用本文： | 赵刚,余其兴,刘江东.黑斑蛙骨髓细胞染色体标本制备的新方法[J].生物学杂志,2010,27(6):97-99. |
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| 作者姓名： | 赵刚 余其兴 刘江东 |
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| 作者单位： | 1. 湖北中医药大学生物学教研室,武汉430065;武汉大学动物分子细胞遗传学实验室,武汉,430072
2. 武汉大学动物分子细胞遗传学实验室,武汉,430072 |
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| 摘 要： | 染色体标本制备是黑斑蛙分子细胞遗传学研究的基础。为了简化操作程序,缩短实验周期,建立了黑斑蛙染色体制备的一种新方法——骨髓细胞体外短时培养与秋水仙素同步处理法。该方法操作简便,重复性较好,可在较短时间里制备出分裂相较多且形态良好的蛙染色体标本,适用于核型分析、染色体荧光原位杂交、染色体显微分离和单染色体文库构建等多个方面的研究。
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| 关 键 词： | 黑斑蛙 染色体制备 新方法 |
Chromosome preparation from bone marrow cells of Rana nigromaculata |
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| ZHAO Gang,YU Qi-xing,LIU Jiang-dong.Chromosome preparation from bone marrow cells of Rana nigromaculata[J].Journal of Biology,2010,27(6):97-99. |
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| Authors: | ZHAO Gang YU Qi-xing LIU Jiang-dong |
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| Institution: | 1.Department of Biology,Hubei University of Chinese Medicine,Wuhan 430065;2.Laboratory of Animal Molecular Cytogenetics,Wuhan University,Wuhan 430072,China) |
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| Abstract: | Chromosome preparation is an essential technique for frog molecular cytogenetics.In order to simplify the experimental procedure,a new method for frog chromosome preparation was established.The key step of this method is that the short-term culture of bone marrow cells treated with colchicine.Result showed that this method is short time-consuming,simple operation and good chromosome morphology. |
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| Keywords: | Rana nigromaculata chromosome preparation new method |
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