| 牡丹试管苗玻璃化的研究 |
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| 引用本文： | 李海刚,孔祥生.牡丹试管苗玻璃化的研究[J].生物学杂志,2010,27(5):35-37,42. |
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| 作者姓名： | 李海刚 孔祥生 |
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| 作者单位： | 河南科技大学农学院,洛阳,471003 |
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| 基金项目： | 洛阳市科技局计划项目 |
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| 摘 要： | 以MS为基本培养基,研究品种、琼脂、6-BA、大量元素、蔗糖、光照等因素对牡丹试管苗玻璃化的影响。结果显示：不同品种的牡丹试管苗在组织培养的过程中,出现不同程度的玻璃化,增加培养基中琼脂浓度、降低细胞分裂素6-BA的浓度、降低大量元素的浓度和增加光照强度或采用自然光照均可以有效地降低试管苗玻璃化。
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| 关 键 词： | 牡丹 试管苗 玻璃化 |
The research on vitrification of Paeonia suffruticosa plantlets |
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| LI Hai-gang,KONG Xiang-sheng.The research on vitrification of Paeonia suffruticosa plantlets[J].Journal of Biology,2010,27(5):35-37,42. |
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| Authors: | LI Hai-gang KONG Xiang-sheng |
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| Institution: | (College of Agronomy,Henan University of Science and Technology,Luoyang 471003,China) |
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| Abstract: | MS medium was chosen as the basic medium and the main factors such as cultivars,agar,6-BA,macroelement,sucrose and intensity of illumination were investigated on vitrification of plantlets from Paeonia suffruticosa.The results showed that different cultivars of Paeonia suffruticosa in tissue culture process appeared different degrees of vitrification,increasing the concentration of agar in culture,reducing the concentration of 6-BA,reducing the concentration of macroelement and the increasing intensity of illumination or using the natural light were possible to reduce the vitrification of plantlets effectively. |
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| Keywords: | Paeonia suffruticosa plantlets vitrification |
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