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| hGH单克隆抗体的筛选及双抗体夹心ELISA检测方法的建立 | |
| 引用本文： | 饶圣宏,刘文,游牧,徐振山,宋礼华.hGH单克隆抗体的筛选及双抗体夹心ELISA检测方法的建立[J].生物学杂志,2011,28(5):94-97. |
| 作者姓名： | 饶圣宏 刘文 游牧 徐振山 宋礼华 |
| 作者单位： | 1. 安徽大学生命科学院,合肥,230039 2. 安徽医科大学生物化学与分子生物学教研室,合肥,230032 3. 安徽安科生物工程(集团)股份有限公司,合肥,230088 |
| 摘 要： | 采用杂交瘤法制备单克隆抗体,并用辛酸-硫酸铵法纯化单抗,通过ELISA方法和Western blotting测定抗体的效价与特异性,并进行抗体类型、相对亲和力测定;应用纯化的单抗建立hGH双抗体夹心ELISA检测方法。筛选出两株可以稳定分泌抗hGH单抗的杂交瘤细胞株,分别命名为3E11、2G9,抗体类型均为IgG1,抗体滴度均可达10-10,特异性好,相对亲和力高,以筛选到的两株单抗建立的双抗夹心ELISA法线性范围为0.09～1.5625ng/mL,R2>0.9,灵敏度为0.09ng/mL。筛选出高效抗hGH的单抗,并建立了hGH双抗体夹心ELISA检测方法。 |
| 关 键 词： | 人生长激素 单克隆抗体 双抗体夹心ELISA法 |
Screening of monoclonal antibody against recombinant human growth hormone and development of double antibody sandwich ELISA for hGH |
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| RAO Sheng-hong,LIU Wen,YOU Mu,XU Zhen-shan,SONG Li-hua.Screening of monoclonal antibody against recombinant human growth hormone and development of double antibody sandwich ELISA for hGH[J].Journal of Biology,2011,28(5):94-97. | |
| Authors: | RAO Sheng-hong LIU Wen YOU Mu XU Zhen-shan SONG Li-hua |
| Institution: | RAO Sheng-hong1,LIU Wen2,YOU Mu2,XU Zhen-shan3,SONG Li-hua3(1.College of Life Science,Anhui University,Hefei 230039,2.Department of Biochemistry and Molecule Biology,Anhui Medical University,Hefei 230032,3.Anhui Anke Biotechnology(group) Co.,Ltd,Hefei 230088,China) |
| Abstract: | To screen the monoclonal antibody(McAb) against recombinant human growth hormone(rhGH) and develop a double antibody sandwich ELISA for hGH,the McAb was prepared by using hybridoma technique and purified by caprylic acid-ammonium sulphate precipitation.The McAB was analyzed for type,titer in ascites,relative affinity and specificity.By using the pair of McAbs,a double antibody sandwich ELISA method was established.Two hybridoma cell strains stably secreting McAb against hGH were screened.The secreted McAbs ... |
| Keywords: | human growth hormone monoclonal antibody(McAb) double antibody sandwich ELISA |
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