荧光Real-time PCR鉴定鸡胸骨总RNA质量

为了更准确地鉴定提取鸡骨总RNA的质量,试验分别用核酸蛋白检测仪、1%琼脂糖凝胶电泳和荧光Real-time PCR检测评价3种不同的方法提取成年鸡胸骨总RNA的质量。结果显示,荧光Real-time PCR可更好地鉴定提取的骨总RNA的质量。用核酸蛋白检测仪、1%琼脂糖凝胶电泳能粗略检测RNA的纯度和完整性,但不能反映提取过程中是否引起基因不均一的减少,所检测的纯度也不能精确反映RNA的反转录效率。

Assessment of total RNA quality from chicken sternum by fluorescence Real-time PCR

In order to accurately analyze the quality and quantity of total RNA from chicken bone, total bone RNA with 3 different extraction methods were detected by Biophotometer plus, 1% agarose gel eleetrophoresis and fluorescent Real-time PCR. The results from Biophotometer plus and 1% agarose gel electrophoresis suggested the three total bone RNA from three different kinds methods could matched with the requirement of experiment. However, the results of real-time PCR showed the total RNA treatment by isopropanol and RNA precipitation solution had excellent quality and quantity. Real-time PCR is a better technique to identify the quality of total bone RNA than Biophotometer plus and 1% agarose ael eleetrophoresis.