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| 日本血吸虫重组信号蛋白14—3—3的纯化及单克隆抗体的制备 | |
| 引用本文： | 曹亮,王弦,谢小强,徐振山,宋礼华.日本血吸虫重组信号蛋白14—3—3的纯化及单克隆抗体的制备[J].生物学杂志,2009,26(2):73-75. |
| 作者姓名： | 曹亮 王弦 谢小强 徐振山 宋礼华 |
| 作者单位： | 1. 安徽大学生命科学学院,合肥,230039 2. 安徽医科大学生物化学与分子生物学教研室,合肥,230032 3. 安徽安科生物工程(集团)股份有限公司,合肥,230088 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 纯化日本血吸虫（中国大陆株）重组信号蛋白（rSj14—3—3），并制备其单克隆抗体。以纯化后的rsj14-3—3蛋白为抗原免疫Balb／c小鼠，用杂交瘤技术制备抗rSj14-3-3的单克隆抗体，并通过ELISA方法和Westernblotting测定抗体的效价与特异性。获得了大量高纯度的rSj14-3-3蛋白：筛选出了能够稳定分泌抗rSj14．3．3单抗的杂交瘤细胞株3H6。单抗亚型为IgG1。实验依靠大肠杆菌表达系统高效表达了rSj14—3—3蛋白，并利用该蛋白制备了单克隆抗体．可用于今后血吸虫病免疫诊断的实验研究。 |
| 关 键 词： | 日本血吸虫 rSj14—3—3蛋白 单克隆抗体 |
Purification of recombinant Schistosoma japonicum signaling protein 14-3-3 and the monoclonal antibody preparation |
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| CAO Liang,WANG Xian,XIE Xiao-qiang,XU Zhen-shan,SONG Li-hua.Purification of recombinant Schistosoma japonicum signaling protein 14-3-3 and the monoclonal antibody preparation[J].Journal of Biology,2009,26(2):73-75. | |
| Authors: | CAO Liang WANG Xian XIE Xiao-qiang XU Zhen-shan SONG Li-hua |
| Institution: | CAO Liang WANG Xian, XIE Xiao-qiang , XU Zhen-shan, SONG Li-hua( 1. College of Life Science, Anhui University, Hefei 230039 ; 2. Department of Biochemistry and Molecule Biology, Anhui Medical University, Hefei 230032; 3. Anhui Anke Bioteehnology (group) Co. , Ltd, Hefei 230088, China) |
| Abstract: | To purify recombinant Schistosoma japonicurn （Chinese strain） signaling protein 14-3-3 and to prepare the monoclonal antibodies （McAbs） against rSj14-3-3, Balb/c mice were immunized with purified rSj14-3-3 protein. The anti-rSj14-3-3 monoclonal antiody was obtained by using hybridoma technique. Characterization of the antibodies was performed by enzyme linked immunosorbent as- say （ELISA） and Western blotting analysis. Results showed that high purified rSj14-3-3 protein and the hybridoma cell line 3H6 secreting monoclonal antibodies （McAbs） against rSj14-3-3 were obtained. The subclass of McAbs was found to be IgG1. The rSj14-3-3 protein is expressed in E. coli cells efficiently and its antibodies were obtained. They can be used in further study about immunodiagnostic methods of Schistosomiasis japonica. |
| Keywords: | Schistosoma japonicum rSj14-3-3 protein monoclonal antibody |
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