假蜜环菌发酵工艺的优化研究

目前,国内普遍采用亮菌固体发酵工艺生产亮菌制剂。采用小型发酵罐液体深层发酵和液体静置发酵对亮菌发酵工艺进行优化研究。液体深层发酵采用28℃,200 r/min,通气量1∶1 v/v·m,培养7 d,亮菌干重为16.55g/L,其中菌丝体中多糖含量为5.42%,蛋白含量为1.75%;液体静置发酵采用500 mL三角瓶,28℃,150 r/min,摇瓶3 d后静置发酵14 d,亮菌干重可达10.69 g/L,发酵液中多糖含量为1.016 g/L,蛋白含量为0.320 g/L。对液体静置发酵进一步研究发现其发酵液中亮菌甲素含量可达3.118 mg/L。由此可见,两种发酵方式在保证生物量和活性成分的前提下,缩短了发酵周期,均优于传统的固体发酵工艺,值得工业生产借鉴。

The optimization of the fermentation technology of Armillariella tabescens

At present,preparation of Armillariella tabescens was generally carried on with solid state fermentation. The optimization of fermentation technology of Armillariella tabescens with taking the deep liquid fermentation of fermentor and stationary liquid fermentation methods was made. The deep liquid fermentation was studied under the condition of 28℃,200 r /min,ventilation of 1 ∶ 1 v /v·m. After culturation for 7 days,the biomass of Armillariella tabescens in dry weight was 16. 55 g /L,and the mycelia contained polysaccharide5. 42%,protein 1. 75%; the stationary liquid fermentation was taken firstly by using 500 mL conical flask to shake for 3 days at 28℃,150 r /min,and then to stationary culture for 14 days,the biomass of Armillariella tabescens in dry weight could reach to 10. 69 g /L,and the fermentation broth included polysaccharide 1. 016 g /L,protein 0. 320 g /L.