纳米ZnO对斑马鱼肝脏生理生化指标的影响

研究了纳米ZnO对斑马鱼肝脏生理生化指标的影响。当斑马鱼暴露在ZnO纳米颗粒浓度为2.8 mg/L、5.6mg/L、11.2 mg/L、22.4 mg/L和44.8 mg/L时,分别测定了6 h、12 h、24 h、48 h和72 h时斑马鱼肝组织中的GSH、MDA、Na＋K＋-ATPase的含量和24 h ROS的变化,结果显示：与对照组相比,处理组中斑马鱼肝组织中GSH的含量显著减少（P〈0.05）,MDA含量随处理浓度的升高却显著增加（P〈0.05）,Na＋K＋-ATPase活性随暴露时间先显著降低后显著升高（P〈0.05）,24 h ROS含量高于对照组（P〈0.05）,说明斑马鱼肝组织的生理活动受到ZnO纳米颗粒的影响,且有时间和浓度依赖性。

Effects of nano ZnO on the physiological and biochemical index in the liver of zebra fish

The effects of nano ZnO on the physiological and biochemical index in the liver of zebra fish were studied in this paper. Zebra fish were exposed to the different concentration（ 8 mg /L,5. 6 mg /L,11. 2 mg /L,22. 4 mg /L and 44. 8 mg /L） of the nano ZnO.The contents of GSH,MDA,and Na＋K＋-ATPase in the liver were detected at 6,12,24,48,and 72 hours. Changes of 24 h ROS were also detected. The results showed that the content of GSH in the liver tissues was significantly lower than that in the control group（ p 0.05）,while the content of MDA in the liver tissues was significantly increased（ p 0.05） with the increasing concentration of nano ZnO. The activity of Na＋/K＋-ATP enzyme in liver tissues was significantly decreased at first,and then increased（ p 0. 05）with a longer exposure time. The content of changes of 24 h ROS in the liver was higher than that in the control group（ p 0. 05）. Experimental results showed that physiology in the liver tissues of zebra fish was affected by the ZnO nanoparticles and also depended on the time and concentration.