| 根霉TC1653产纤维素酶提纯及酶学性质研究 |
| |
| 引用本文： | 陈阿娜,汤斌,张庆庆,陈中碧.根霉TC1653产纤维素酶提纯及酶学性质研究[J].生物学杂志,2009,26(4):21-23. |
| |
| 作者姓名： | 陈阿娜 汤斌 张庆庆 陈中碧 |
| |
| 作者单位： | 安徽工程科技学院生物化学工程系,芜湖,241000 |
| |
| 基金项目： | 安徽工程科技学院青年科研基金项目 |
| |
| 摘 要： | 对根霉所产纤维素酶酶系进行了分析并研究了部分酶学性质。实验选择超滤和凝胶柱分离相结合的方式提纯纤维素酶，结果显示根霉TC1653纤维素酶系是一个完全酶系，具有一个较为明显的内切葡聚糖酶组分。β-葡萄糖苷酶组分的最适反应温度为70℃，温度高于70℃时，活性迅速下降，但在这种高温下具有最高反应活性的酶很少见，很可能又是一种新的β-葡萄糖苷酶。
|
| 关 键 词： | 根霉 纤维素酶 提纯 酶学性质 |
Study on purification and properties of cellulases produced by Rhizopus sp. TC1653 |
| |
| CHEN A-ha,TANG Bin,ZHANG Qing-qing,CHEN Zhong-bi.Study on purification and properties of cellulases produced by Rhizopus sp. TC1653[J].Journal of Biology,2009,26(4):21-23. |
| |
| Authors: | CHEN A-ha TANG Bin ZHANG Qing-qing CHEN Zhong-bi |
| |
| Institution: | ( Department of Biochemical Engineering, Anhui University of Science and Technology, Wuhu 241000, China) |
| |
| Abstract: | Uhrafiltration and sephadex G-100 were used to purify cellulases produced by TC1653. The results showed that the cellulases produced by TC1653 were a complete enzyme system and one kind of endo-1,4-β-D-glucanase was obtained. The optimal temperature of β-glucosidase was 70℃. The β-glucosidase enzyme activity would be rapidly dropped when the temperature was higher than 70℃. It may be a new kind of β-glucosidase, though at such a high temperature with the highest activity is rare. |
| |
| Keywords: | Rhizopus sp cellulase purification enzymatic properties |
| 本文献已被 维普 万方数据 等数据库收录！ |
