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| 低氧条件下大鼠血管内皮细胞的形态观察 | |
| 引用本文： | 苗兰英,林大勇,白剑,杨葳,张伯阳,王良君,杨菁,刘书林.低氧条件下大鼠血管内皮细胞的形态观察[J].生物学杂志,2011,28(4):18-20,30. |
| 作者姓名： | 苗兰英 林大勇 白剑 杨葳 张伯阳 王良君 杨菁 刘书林 |
| 作者单位： | 1. 辽宁中医药大学实验技术中心,沈阳,110847 2. 辽宁中医药大学实验技术中心,沈阳,110847;中国医科大学实验动物部,沈阳,110001 3. 中国医科大学实验动物部,沈阳,110001 4. 辽宁医学院,辽宁,锦州,110021 |
| 基金项目： | 辽宁省自然科学基金资助项目 |
| 摘 要： | 原代培养大鼠血管内皮细胞,将培养箱内通入5%CO2-95%N2混合气体（氧分压为18.3 mmHg）并培养大鼠血管内皮细胞12、24h,使用MTT法、LDH活力测定及细胞骨架染色对低氧细胞模型鉴定,研究大鼠血管内皮细胞低氧模型的建立条件及其形态学特点。在低氧12h条件下,血管内皮细胞存活率降低、LDH释放增加,但细胞骨架保持完整;在低氧24h条件下,血管内皮细胞存活率降低、LDH释放增加,细胞骨架破碎。结果表明在低氧（氧分压为18.3 mmHg）24h条件下,可以建立大鼠血管内皮细胞低氧模型。 |
| 关 键 词： | 血管内皮细胞 低氧 动物模型 细胞骨架 |
The morphological observation of hypoxic rat model in vascular endothelial cells |
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| MIAO Lan-ying,LIN Da-yong,BAI Jian,YANG Wei,ZHANG Bo-yang,WANG Liang-jun,YANG Jing,LIU Shu-lin.The morphological observation of hypoxic rat model in vascular endothelial cells[J].Journal of Biology,2011,28(4):18-20,30. | |
| Authors: | MIAO Lan-ying LIN Da-yong BAI Jian YANG Wei ZHANG Bo-yang WANG Liang-jun YANG Jing LIU Shu-lin |
| Institution: | MIAO Lan-ying1,LIN Da-yong1,BAI Jian1,2,YANG Wei2,ZHANG Bo-yang2,WANG Liang-jun3,YANG Jing3,LIU Shu-lin3(1.Liaoning University of Traditional Chinese Medicine,Liaoning Shenyang,110847,2.The Laboratory Animal Centre,China Medical University,Shenyang 110001,3.Liaoning Medical University,Jinzhou 110021,China) |
| Abstract: | To research on establishment conditions of hypoxic rat model in vascular endothelial cells and its morphological characteristics,primary cultured rat vascular endothelial cells were incubated for 12 hours and 24 hours in the 5%CO2-95%N2 mixed air(the 18.3 mmHg partial pressure of the oxygen).The hypoxia model was determined by MTT assay,lactate dehydrogenase(LDH) activity and cytoskeleton stain.When the vascular endothelial cells were cultured under hypoxia for 12 hours,the survival rate of vascular endothe... |
| Keywords: | the vascular endothelial cells hypoxic animal model cytoskeleton |
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