Molecular cloning and expression analysis of chymotrypsin-like serine protease from the redclaw crayfish (Cherax quadricarinatus): A possible role in the junior and adult innate immune systems |
| |
Authors: | Di-An Fang Xian-Ming Huang Zhi-Qin Zhang Dong-Po Xu Yan-Feng Zhou Min-Ying Zhang Kai Liu Jin-Rong Duan Wei-Gang Shi |
| |
Institution: | 1. Scientific Observing and Experimental Station of Fishery Resources and Environment in the Changjiang River, Freshwater Fisheries Research Center, Shanshui Road 9, Wuxi 214081, China;2. Freshwater Fisheries Research Institute of Zhejiang Province, Hang Chang Qiao Road 116, Huzhou 313001, China;3. Shangrao Normal University, Zhiming Road 88, Shangrao 334001, Jiangxi Province, China |
| |
Abstract: | A novel chymotrypsin-like serine protease (CLSP) was isolated from the hepatopancreas of the redclaw crayfish Cherax quadricarinatus (Cq-chy). The full-length cDNA of Cq-chy contains 951 nucleotides encodes a peptide of 270 amino acids. The mature peptide comprising 223 amino acids contains the conserved catalytic triad (H, D, and S). Similarity analysis showed that Cq-chy shares high identity with chymotrypsins from the fiddler crab; Uca pugilator. Cq-chy mRNA expression in C. quadricarinatus was shown to be: (a) tissue-related with the highest expression in the hepatotpancreas and widely distributed, (b) highly responsive in the hepatopancreas to White Spot Syndrome Virus (WSSV) challenge, and (c) differently regulated in immature and adult crayfish. In this study we successfully isolated Cq-chy. Our observations indicate that Cq-chy is differently involved in the immature and adult innate immune reactions, thus suggesting a role for CLSPs in the invertebrate innate immune system. |
| |
Keywords: | Chymotrypsin-like serine protease The innate immune system |
本文献已被 ScienceDirect 等数据库收录! |
|