| MNNG转化赤麂细胞株（KIZ—8401）的生长特性和核型观察 |
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| 引用本文： | 刘爱华,林世英.MNNG转化赤麂细胞株（KIZ—8401）的生长特性和核型观察[J].动物学研究,1992,13(1):77-82. |
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| 作者姓名： | 刘爱华 林世英 |
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| 作者单位： | 中国科学院昆明动物研究所 650107
(刘爱华，林世英)，中国科学院昆明动物研究所 650107(熊习昆) |
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| 摘 要： | 本文用MNNG(N-Methyl-N'-nitro-N-nitrosoguanidine)(甲基硝基亚硝基胍)转化赤麂肺成纤维细胞株(KIZ-7901)。转化的赤麂细胞株(KIZ-8401)具有新的核型,2n=8♂,细胞形态,细胞周期以及生物学特性都与未转化的细胞株(2n=7♂)不一样。转化细胞中还常见多倍体出现,细胞具有克隆生长能力。
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| 关 键 词： | MNNG 转化 赤麂细胞 核型 |
OBSERVATIONS ON NEW CELL LINE OF Indian muntjac TRANSFORMED WITH MNNG |
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| Liu Aihua Lin Shiying Xiong Xikun.OBSERVATIONS ON NEW CELL LINE OF Indian muntjac TRANSFORMED WITH MNNG[J].Zoological Research,1992,13(1):77-82. |
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| Authors: | Liu Aihua Lin Shiying Xiong Xikun |
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| Abstract: | Lung fibroblast line of Indian muntjac (KIZ-7901) was transformed with MNNG (N-Methyl-N-nitro-N-nitrosoguanidine). The transformed Indian mu-ntjac: cells (KIZ-8401) possessed the new karyotype with cbromorsome number of 8 in male and polyploid. The morphology and cell cycle as well as mitotic index in MNNG-transformed muntjac cells were different from diploid cell line of Indian muntjac (KIZ-7901). Moreover, the MNNG-transformed muntjac cells possessed colony-forming capacity in vitro. |
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| Keywords: | MNNG Transformation Indian muntjac cell line Karyotype |
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