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微卫星评价牙鲆雌核发育二倍体纯合性
引用本文:朱晓琛,刘海金,孙效文,薛玲玲,毛连菊.微卫星评价牙鲆雌核发育二倍体纯合性[J].动物学研究,2006,27(1):63-67.
作者姓名:朱晓琛  刘海金  孙效文  薛玲玲  毛连菊
作者单位:1. 中国水产科学院黑龙江水产研究所,黑龙江,哈尔滨,150070;大连水产学院,生命科学与技术学院,辽宁,大连,116023
2. 中国水产科学院黑龙江水产研究所,黑龙江,哈尔滨,150070
3. 大连水产学院,生命科学与技术学院,辽宁,大连,116023
基金项目:国家科技攻关项目(96-920-44-01)
摘    要:采用8个微卫星座位分别对牙鲆减数雌核发育二倍体家系和卵裂雌核发育二倍体家系的纯合性进行检验。卵裂雌核发育二倍体在所有检测座位全部纯合。减数雌核发育二倍体在部分座位发生纯合,但未发现在所有座位全部纯合的个体,在Poli9TUF、Poli9-8TUF、Poli11TUF、Poli13TUF、Poli23TUF、Poli30TUF、Poli123TUF和Poli130TUF座位,杂合子比例分别为1·0000、1·0000、0·1944、0·9459、0·8611、1·0000、0·7778和0·8000,平均杂合子比例为0·8224。由此表明,牙鲆除了Poli11TUF外,在其余7个座位均具有很高的重组率。研究结果显示,牙鲆卵裂雌核发育二倍体一代即可形成纯合子;而减数雌核发育二倍体由于具有较高的重组率,使其与母本的遗传同质性较高。

关 键 词:牙鲆  雌核发育  纯合性  微卫星
文章编号:0254-5853(2006)01-0063-05
收稿时间:2005-11-07
修稿时间:2005-11-072005-12-26

Assessment of Homozygosity in Gynogenetic Diploid Using Microsatellite Markers in Japanese Flounder (Paralichthys olivaceus)
ZHU Xiao-chen,LIU Hai-jin,SUN Xiao-wen,XUE Ling-ling,MAO Lian-ju.Assessment of Homozygosity in Gynogenetic Diploid Using Microsatellite Markers in Japanese Flounder (Paralichthys olivaceus)[J].Zoological Research,2006,27(1):63-67.
Authors:ZHU Xiao-chen  LIU Hai-jin  SUN Xiao-wen  XUE Ling-ling  MAO Lian-ju
Institution:1. Heilongfiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 2. Life Science and Technology Institute, Dalian Fisheries University, Dalian 116023, China
Abstract:Gynogenesis was thought to be a useful method to generate inbred lines in fish. In this study, analysis of 5-8 heterozygous loci in Japanese flounder (Paralichthys olivaceus) was used to measure the increment of homozygosity in meiogynogenetic diploid (Meiotic-A & Meiotic-B) and mitogynogenetic diploid (Mitotic-A). The result showed all homozygosity in Mitotic-G1, while a high percentage of heterozygosity in Meiosis-A and Meiosis-B except Poli11TUF. The rate of heterozygosity in meiogynogenetic progenies at the locus of Poli9TUF, Poli9-8TUF, Poli11TUF, Poli13TUF, Poli23TUF, Poli30TUF, Poli123TUF and Poli130TUF were 1.000 0, 1.000 0, 0.194 4, 0.945 9, 0.861 1, 1.000 0, 0.777 8 and 0.800 0, respectively. The average recombination rate of these eight loci is 0.822 4. The high proportion of heterozygotes for seven loci demonstrates that it is not a practical method for producing homozygous inbred lines in the gynogenetic fish produced by retention the second polar body; and treatments of suppressing the first cell division were more promising for this purpose.
Keywords:Japanese flounder  Gynogenesis  Homozygosity  Microsatellite marker
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