| 随机扩增多态DNA影响因素的研究 |
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| 引用本文： | 陈永久,张亚平.随机扩增多态DNA影响因素的研究[J].动物学研究,1997,18(2):221-227. |
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| 作者姓名： | 陈永久 张亚平 |
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| 作者单位： | 中国科学院昆明动物研究所细胞与分子进化开放研究实验室 |
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| 摘 要： | 随机拉多态ＤＮＡ分析受诸多因素的影响，我们发现不同厂家制造的ＰＣＲ扩增仪，不同厂家出品的ＴａｑＤＮＡ聚合酶和ＰＣＲ缓冲液，ＲＡＰＤ反应体系中的引物浓度，Ｍｇ＾２＋浓度，ｄＮＴＰ浓度，ＢＳＡ和明胶，以及模板ＤＮＡ的量等均可能对ＲＡＰＤ结果有不同程度的影响。
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| 关 键 词： | DNA 检测 RAPD 影响因素 |
STUDIES ON FACTORS IN RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS |
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| Chen Yongjiu,Zhang Yaping.STUDIES ON FACTORS IN RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS[J].Zoological Research,1997,18(2):221-227. |
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| Authors: | Chen Yongjiu Zhang Yaping |
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| Abstract: | In this paper, we studied factors which may affect the results of random amplified polymorphic DNA. We found that different PCR amplifier, TaqDNA polymerase, and concentration of Mg 2+ , dNTP, BSA, gentin, and template DNA may result in different RAPD results. To make the RAPD data more repeative and comparitive, we recommand that those factors should be clearly stated in all the paper. |
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| Keywords: | RAPD Affected facters Standardization |
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