四种杀虫剂对两种书虱羧酸酯酶和乙酰胆碱酯酶的抑制作用

选用有机磷类杀虫剂(敌敌畏、毒死蜱、对氧磷)和氨基甲酸酯类杀虫剂(丁硫克百威)，通过生物测定(药膜法)和生化测定(比色法)比较了嗜卷书虱和嗜虫书虱对所选药剂的敏感差异性。根据LC50可知嗜虫书虱对所选药剂比嗜卷书虱敏感。离体酶活性分析结果显示嗜卷书虱和嗜虫书虱的羧酸酯酶只对敌敌畏敏感，且嗜卷书虱比嗜虫书虱更敏感；4种药剂对乙酰胆碱酯酶均有强烈的抑制作用，同样是嗜卷书虱比嗜虫书虱敏感。乙酰胆碱酯酶的动力学研究结果和离体酶活性测定相一致。聚丙烯酰胺凝胶电泳分析显示，4种杀虫剂离体条件下对2种书虱的酯酶同工酶的抑制能力有明显差异，其中敌敌畏的抑制力最强；但对不同同功酶的抑制趋势(对大分子的抑制似乎较强)是一致的。酶的敏感性分析结果与生测结果比较表明，2种书虱的耐药力差异与其乙酰胆碱酯酶和酯酶对药剂的敏感性无关。如要弄清耐药力机制，需做进一步研究。

Inhibition of Carboxylesterase and Acetylcholinesterase in Liposcelis bostrychophila and L.entomophila (Psopotera:Liposcelididae) by Four Inhibitors

The inhibition of carboxylesterase (CarE) and acetylcholinesterase (AChE) in booklices by dichlorvos,paraoxon,chlorpyrifos and carbosulfan was studied.The results of bioassay revealed that these insecticides showed strong inhibition to Liposcelis spp.,and L.bostrychophila was less sensitive than L.entomophila.CarE of L.bostrychophila was more susceptible to inhibition than that of L.entomophila only to dichlorvos.For the AChE,the four inhibitors showed strong inhibition to both L.bostrychophila and L.entomophila and had similar susceptibility tendency with CarE;and inhibition kinetics also indicated the similar tendency.The inhibition pattern for two species was simply tested by PAGE and it was obvious that the endurance of two psocids to these insecticides has no direct relevence with the sensitivity of CarE and AChE.The further study should be done to realize the real mechanism of endurance.