变色树蜥消化道5-羟色胺细胞的免疫组织化学定位

用免疫组织化学ABC法,应用5-羟色胺(5-HT)特异性抗血清,对变色树蜥(Calotes versicolor)消化道内含有5-HT的内分泌细胞进行了免疫组织化学的定位研究和形态学观察。结果显示,5-HT细胞在变色树蜥消化道的各个部位均有分布,分布密度近似呈“M”形,其中以空肠分布密度最高,胃体次之,食管最低。5-HT细胞的形态多样,呈圆形、椭圆形、锥体形、长锥体形等,其中胃、幽门和直肠以圆形和椭圆形为主,小肠则以长锥体形为主,其细长突起指向肠腔或固有膜;细胞分布于上皮基部、上皮细胞之间、腺泡上皮细胞之间或固有膜内;多数细胞以内分泌功能为主,少数细胞具有外分泌功能。比较分析表明,5-HT细胞分布型可能与动物的食性有关。

Immunohistochemical Localization on 5-HT Cells in the Digestive Tract of Calotes versicolor

The presence investigation and morphology observation of 5-HT endocrine cells in the digestive tract of Calotes versicolor were done with the anti-5-HT serum by the method of the ABC immunohistochemical technique.The results indicate that 5-HT cells distribute throughout the digestive tract,and the density curve of 5-HT cells appears "M".The distributive density of 5-HT cells is the maximum in jejunum,the second in stomach and the minimum in esophagus.The shapes of 5-HT endocrine cells are diverse,such as round,ellipse,shuttle,tapering and so on.The cells are mainly round and ellipse in the stomach,pyloric part of stomach and rectum,while the cells are mainly tapering in the small intestine and the tapering cells always have one long processus extending to either the gut lumen or lamina propria.The 5-HT cells are found between gastrointestinal epithelial cells,at the basal region of epithelial cells,between glandular epithelial cells and in the lamina propria.The majority of 5-HT endocrine cells perform endocrine function and the minority has exocrine function.It indicated that the distribution of 5-HT cells may be related to the feeding habit of Calotes versicolor by analysing and contrasting.