首页 | 本学科首页   官方微博 | 高级检索  
   检索      

猫视网膜年龄相关的形态学变化
引用本文:孙庆艳,梅斌,王海涛,朱再满,张长征,罗勋,华田苗,刘再群.猫视网膜年龄相关的形态学变化[J].动物学研究,2004,25(6):538-542.
作者姓名:孙庆艳  梅斌  王海涛  朱再满  张长征  罗勋  华田苗  刘再群
作者单位:安徽师范大学,生命科学院,安徽,芜湖,241000
基金项目:安徽省自然科学基金 (982 41716),安徽省教育厅科研基金 (2 0 0 3kj15 2,2 0 0 4kj177),安徽省重点实验室科研基金,安徽师范大学青年基金 (2 0 0 4xqn0 9)资助项目
摘    要:取老年猫(12龄,3~3.5kg)和青年猫(1~3龄,2~2.5kg)各4只的视网膜,经4%多聚甲醛处理后,用H.E.染色以显示视网膜结构,Nissl染色显示神经节细胞,免疫组织化学ABC法染色以显示星形胶质细胞特征性标志物胶质纤维酸性蛋白(GFAP)的阳性反应细胞的分布。显微镜下观察测量视网膜厚度,计数神经节细胞、GFAP免疫反应阳性细胞数。与青年猫比较,老年猫视网膜总厚度以及外核层、外网状层、内核层和内网状层厚度均显著减小;神经节细胞层的细胞密度显著下降;GFAP免疫反应阳性细胞显著增加,GFAP阳性细胞阳性反应强,胞体明显膨胀,突起稠密粗大。推测在衰老过程中视网膜细胞有神经元丢失现象,可能是造成视觉功能衰退的重要原因之一;视网膜星形胶质细胞的功能增强可能会延缓衰老。

关 键 词:视网膜  GFAP  神经节细胞  阳性细胞  星形胶质细胞  老年  免疫反应  衰老过程  阳性反应  镜下观察
文章编号:0254-5853(2004)06-0538-05

Age-related Morphological Changes in the Retina of Cat
SUN Qing-yan,MEI Bin,WANG Hai-tao,ZHU Zai-man,ZHANG Chang-zheng,LUO Xun,HUA Tian-miao,LIU Zai-qun.Age-related Morphological Changes in the Retina of Cat[J].Zoological Research,2004,25(6):538-542.
Authors:SUN Qing-yan  MEI Bin  WANG Hai-tao  ZHU Zai-man  ZHANG Chang-zheng  LUO Xun  HUA Tian-miao  LIU Zai-qun
Institution:College of Life Sciences,Anhui Normal University,Wuhu,Anhui 241000,China
Abstract:The retinae of 4 old cats (12 years,3-3.5 kg) and 4 young cats (1-3 years,2-2.5 kg) were selected.After treated in 4% paraformaldehyde,we applied Hematoxylin-Eosin and Nissl staining to exhibit the structure of the retinae and ganglion cells respectively,and also we used immunohistochemical ABC method to display the distribution of GFAP-immunoreactive (GFAP-IR) cells that were specifically classified as the astrocytes.By microscope we observed the thickness of retinae,counted the numbers of ganglion cells and GFAP-IR cells.Compared with young cats,the retinae in old cats presented a significant decrease in the thickness of total retina,outer nuclear layer,outer plexiform layer,inner nuclear layer,inner plexiform layer as well as an evidently lower density of ganglion cells.Also old cats showed clearly larger number and stronger staining of GFAP-IR cells than did in young cats,and at the same time the GFAP-IR cell bodies and dendrites swelled obviously in old cats.We presume that neurons lose in old cats,which hints that the loss of retinal cells might be of an important factor mediating visual functional declines during senescence.The functional increase of astrocytes may suspend more aging.
Keywords:Cat  Retina  Age  Morphological change  Astrocyte  GFAP  
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《动物学研究》浏览原始摘要信息
点击此处可从《动物学研究》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号