中国林蛙婚垫的显微结构变化及其与睾酮的关系

用光镜观察中国林蛙（Rana chensinensis）婚垫显微结构的年周期变化，并对婚垫内的睾酮（T）和雄激素受体（AR）进行免疫细胞化学检测，其结果表明：婚刺的形成是由生发细胞增生、颗粒细胞数量增多和体积增大所致，其消退是由角质细胞包裹的颗粒细胞小体脱落所致。婚腺发育与婚刺形成同步，在林蛙婚配期间婚腺的分泌活动最旺盛。T和AR在颗粒细胞、生发细胞和婚腺内的分布与婚垫显微结构的变化周期一致，说明婚垫的形成与消退受T调控。婚垫发育的变化剧期与精子发生进程和精巢系数具相关性，即婚垫的形态特征可间接反应精巢的发育状况．

Correlation Between the Changes of Microstructure and Testosterone in Nuptial Pad of the Frog, Rana chensinensis

The nuptial pad microstructures of Rana chensinensis in the Qinling Mountain China were observed by light microscopy during the annual reproductive cycle; meanwhile, testosterone (T) and androgen receptor (AR) localization were examined by immunocytochemistry in the nuptial pad. The results indicated that the formation of nuptial spines was caused by multiplication of germinal cells, granular cells, and volume expansion of granular cells. The regression of nuptial spines was caused by desquamation of granular cell globules, which wrapped by a layer of horny cells and detaching from epidermis surface. The development of nuptial glands synchronized with nuptial spines morphogenesis. Secretory activity of the nuptial glands was active during courtship. The distribution of T and AR in germinal cells, granular cells and nuptial glands showed a good agreement with aspection of microstructures in nuptial pad. These indicated that the formation and regression of nuptial pad were regulated by T. Seasonal variation in the development of nuptial pad were associated with testis somatic index and spermatogenesis progress. It suggested that pad morphological characters reflected indirectly the development condition of testis.