黑鲷精子的超低温冻存及DNA损伤的SCGE检测

以0.5 mL的麦细管为冻存管和DMSO为抗冻剂进行超低温冷冻黑鲷精子，对冻精核DNA的损伤情况进行单细胞凝胶电泳（SCGE）检测，其结果表明，以Cortland溶液为稀释液，5%、10%、15%及20%DMSO为抗冻剂的超低温冻存的黑鲷精子活力、受精率与鲜精无显著差异。其中以10%DMSO为抗冻剂的冻存效果最佳，冻精的激活率、运动时间、寿命及受精率分别达（92.91±1.25）%、（39.90±2.70）min、（53.82±2.84）min及（89.35±1.99）%；而以25%及30%DMSO为抗冻剂时，冻精活力及受精率显著下降。SCGE检测结果显示，DMSO浓度为5%、10%、15%及20%时，黑鲷冻精与鲜精的彗星率及损伤系数差异不显著；DMSO浓度为25%及30%时，冻精与鲜精的彗星率及损伤系数差异显著；冻精的彗星率与抗冻剂DMSO浓度成正相关。黑鲷鲜精及冻精核的DNA损伤主要为轻度和中度损伤，重度损伤比例较低，完全损伤仅存在于25%及30%DMSO为抗冻剂的冻精中，且比例低。分析认为，较高浓度的DMSO是引起冻精核DNA损伤的主要原因。

Sperm Cryopreservation in Sparus macrocephalus and DNA Damage Detection with SCGE

In this paper, DMSO was used as cryoprotectant for cryopreservation of Sparus macrocephalus spermatozoa in 0.5 mL straws. Detection of DNA damage in response to a cryopreservation process in Sparus macrocephalus spermatozoa was also carried out. The results demonstrated that there were no significant differences between frozen-thawed sperm conserved by Cortland solution diluted with 5%, 10%, 15%, 20% DMSO and fresh sperm in motility. The best motility of frozen-thawed sperm were obtained when DMSO concentra...