交配行为对雄性棕色田鼠雌激素及雌激素β受体在相关脑区的影响

应用行为观察、放射免疫分析和免疫组织化学相结合的方法,研究了雄性棕色田鼠在交配后血清中的雌二醇(E)、与交配行为有关的脑区E免疫阳性细胞数目(E-IRs)、雌激素β受体(ERβ)免疫阳性细胞数目(ERβ-IRs)的变化.将睾丸下降的成年雄性棕色出鼠分成3组:(1)对照组:嗅闻24h新鲜锯木.(2)暴露组:嗅闻24h动情期雌鼠底物.(3)交配组:与动情期雌鼠交配24h.放射免疫榆测血清中的E浓度,交配组比暴露组、对照组显著增高,暴露组和对照组无显著差异.通过免疫组化检测与性行为有关的脑区:弓状核(ARC)、终纹床核(BST)、隔外侧核(LS)、杏仁内侧核(ME)、内侧视前区(MPO)、下丘脑腹内侧核(VMH)E-IRs和ERβ-IRs,E-IRs在交配组比对照组和暴露组各区域都显著增多,暴露组比对照组在隔外侧核显著增多外,其他区域无显著差异.

Changes of Estrogen in Serum and Estrogen Receptor β in the Relevant Brain Regions Following Mating Behavior of the Male Mandarin Vole Microtus mandarinus

In order to investigate the estrogen and estrogen receptor β changes after mating behavior of male mandarin vole (Microtus mandarinus), the radioimmunoassay (RIA) and immunohistochemistry methods were used to investigate changes of the serum estrogen (E) concentrations, estrogen immunoreactive neurons (E-IRs) and estrogen receptor β immunoreactive neurons (ERβ-IRs) in the relevant brain regions following mating behavior. Fifteen sexually matured male voles were randomly divided into three groups and treated differently: (1) control group: voles were exposed to clean hard-wood shavings (n=5), (2) exposure group: voles were exposed to the soiled bedding for more than 24h on which estrous females had been placed (n=5), and (3) mating group: voles were placed with an estrous female for more than 24h (n=5). The results showed circulating serum E concentrations were significantly higher in the mating group than in the exposure group and the control group, and there were no significant difference between the exposure group and the control group. E-IRs and ERβ-IRs were detected in the following brain regions related to mating behavior: the arcuate nucleus (ARC), bed nucleus of the stria terminalis (BST), lateral septal nucleus (LS), medial amygdaioid nucleus (ME),medial preoptic area (MPO) and ventromedial hypothalamic nucleus (VMH). The results showed that there were significantly more E-IRs in the six brain regions in the mating group than in the control group and the exposure group, and there were no significant difference between the exposure group and the control group except for LS. There was no significant difference in ERβ-1Rs in the six brain regions among the three groups, and there were some lighter -stained ERβ-IRs in these brain regions. The results suggested that estrogen affect mating activity of male mandarin voles, hut ERβ might not play an important role in mating behavior of male mandarin voles. Instead, it might be through other receptors.