黑龙江省强壮水螅遗传多样性的RAPD分析

应用RAPD技术对黑龙江省10个分布区17个种群的强壮水螅(Hydrarobusta)进行了遗传多样性分析。从80个随机引物中筛选出19个引物,共扩增出320条带,分子量在0.3～3.0kb,其中多态性带数299个,占93.44%,表现出高度的遗传多样性。17个种群的遗传相似系数(GI)平均值为0.7158,相应的遗传距离(GD)平均值0.3425;分布区内种群间的差异(GD平均值0.1695)小于各分布区间种群间的差异(GD平均值0.3425),各种群间的遗传距离与它们的地理间距基本一致;但虎头种群与其他种群间的相似度明显偏低(GI平均值0.5413,GD平均值0.6161),视为较特化种群。

Genetic Diversity of Hydra robusta in Heilongjiang Province by RAPD Analysis

The genetic diversity of Hydra robusta was studied by RAPD technique from 17 populations of 10 distribution areas in Heilongjiang Province. Nineteen primers were selected from 80 random primers, and yielded a total of 320 bands ranging from 0.3-3.0 kb, 299 (93.44%) of which were polymorphic. The result indicated high level of genetic diversity in this species. The values of genetic identity (GI) and genetic distance (GD) of those populations were 0.7158 and 0.3425 on average, respectively. GD within distribution areas (0.1695) was lower than that between (0.3425). Cluster analysis showed that GDs of populations were consistent with their geographic distances. The population of Hutou had a clearly lower genetic identity with others (GI=0.5413 and GD=0.6161 on average) and was considered a relatively specialized one.