2004年初广东省高致病性禽流感(H5N1)疫点野生鸟类禽流感研究简报

2004年初禽流感疫情期间，针对广东省疫点地区开展了野生鸟类禽流感病毒（H5、H7、H9）调查取样工作，共获得血样76份、拭子125份、器官样21份。血清检测采用琼脂扩散（AGP）和血凝抑制试验（H1），病毒检测用鸡胚法鉴定。分析结果：（1）只在非疫点的汕头南澳的八哥上检测到H9病毒；（2）雷州、罗定、海丰及汕头4个地区的血清检测结果中H5和H9阳性率分别为31．6％和23．7％；沿海地区阳性率极显著高于非沿海地区；（3）候鸟的阳性率极显著高于留鸟。因此，该次疫情中候鸟中存在传播禽流感病毒的可能性。

Preliminary Investigation of Avian Influenza in Wild Birds in the Highly Pathogenic Avian Influenza Infected Regions of Guangdong in Early 2004

We sampled wild birds for the Avian Influenza Virus (AIV)(H5, H7 and H9) survey in the AI infected regions of Guangdong during its eruptive period in early 2004. Seventy-six samples of blood serum, 125 swab samples and 21 organic samples were collected. The method of agar gel precipitation (AGP) and hemagglutination inhibitory (HI) was adopted in serum detection. The chicken embryo culture was adopted in virus detection. Our results showed: (1) H9 viruses were only found in starlings of Nan'ao, Shantou,in the AI noninfected regions.(2) 31.6% H5 and 23.7% H9 immunoreactive blood serums were detected in Leizhou, Luoding, Haifeng and Shantou. The positive rates of wild birds in inshore area are higher than in other regions. (3) The positive rate of migratory birds is higher than resident birds. Therefore, we concluded that migratory birds would spread AIV in its eruptive period.