滇池高背鲫和方正银鲫酯酶、乳酸脱氢酶同工酶的比较研究

本研究以方正银鲫(Carassius auratus gibelio Bloch)普通鲫(Carassius auratus)和滇池高背鲫(Carassius sp.)的各种组织器官为材料,进行酯酶(Esterase)和乳酸脱氢酶(LDH)同工酶电泳图谱的分析比较。结果表明:9种不同组织中酯酶同工酶谱带各不相同,有明显的组织特异性。滇池高背鲫的酯酶谱图有3种表型。方正银鲫和滇池高背鲫同一组织的LDH同工酶酶谱也有明显差异。等电聚焦凝胶电泳(T=7.5％,C=5％)的结果又表明这二种鱼的肝脏、脑、卵的酯酶同工酶酶谱及电泳扫描图亦有差异。这些结果揭示滇池高背鲫与方正银鲫至少在生化水平上已有明显的分化,很可能起源于不同的地区,由不同的祖先,独立演化而形成。滇池高背鲫与云南普通鲫的LDH酶谱较为接近,这说明滇池高背鲫最可能起源于云南本地的普通鲫。

COMPARATIVE STUDIES ON THE ELECTROPHOREGRAM OF ESTERASE ISOZYME AND LACTATE DEHYDROGENASE OF CARASSIUS AUKATUS GIBELIO BLOCH AND CARASSIUS SP.

The Isozyme patterns and relative mobile characteristic of lactate dehydroge-nase (LDH) and esterase from various organs and tissues of Carassius auratus gibelio Bloch, Carassius auratus, Carassius sp., have been comparatively studied by vertical polyacrylamide electrophoresis method. Specific isozyme patterns were observed in the serum, lence, brain, heart, kidney, spleen, liver, muscle, ovary of Carassius sp. The results showed significant tissue-specific difference in esterase zymograms. There are 3 types of phenotypes. Our results indicated that zymogram for LDH isozyme activity in the same organs of Carassius auratus gibelio Bloch and Carassius sp. showed significant differences in band numbers and their strength and relative mobilities. The results of disc polyacrylamide gel isoelectric focusing (T 7.5%, C 5 %) showed that zymogram and electrophoresis densitometric scans for Est. isozyme in the same organs between the two kinds of fishes displayed clear differences (liver, ovary, brain). These results showed some differentiation already at biological level in Carassius auratus gibelio Bloch and Carassius sp. They possibly originated from different regions and developed independently from different ancestors. LDH zymograms of Carassius sp. and Carassius auratus were similar. It seems that Carassius sp. originated possibly from native Carassius auratus of Yunnan Province.