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贵州地方山羊品种的RAPD分析
引用本文:陈祥,廖正录,李国红,张芸,简承松,魏泓,李虹.贵州地方山羊品种的RAPD分析[J].动物学研究,2004,25(2):141-146.
作者姓名:陈祥  廖正录  李国红  张芸  简承松  魏泓  李虹
作者单位:1. 贵州大学,动物科学学院,贵州,贵阳,550025
2. 贵州省农业厅畜禽品种改良站,贵州,贵阳,550001
3. 贵州师范大学,生物系,贵州,贵阳,550001
4. 第三军医大学,实验动物中心,重庆,400038
5. 贵州省水城县畜牧局,水城,553000
基金项目:贵州大学校基金(2 0 0 0 ),贵州省科学技术基金 [黔基合计字(2 0 0 2 ) 3 0 5号 ],国家自然科学基金资助项目(3 0 0 70 12 0 )
摘    要:用180条引物对黔东南小香羊、贵州白山羊、贵州黑山羊和黔北麻羊4个贵州地方山羊品种(种群),以及南江黄羊和波尔山羊进行RAPD分析,其中27条引物扩增出多态性图谱。这27条引物共扩增出281条带,多态带为115条,平均多态频率为40.92%(范围20%~80%);每条引物平均扩增条带为10.41条(范围4~16条);扩增带分子量在210~2800bp。贵州白山羊与贵州黑山羊之间的遗传距离指数(0.0605)最小,而波尔山羊与其他品种之间的遗传距离指数(0.1059~0.1488)最大。NJ法聚类结果显示,贵州白山羊与贵州黑山羊间的亲缘关系最近,其次为黔北麻羊,而黔东南小香羊与其他3个贵州地方品种的亲缘关系较南江黄羊还远。分析表明,黔东南小香羊在遗传上为一独立的品种;而贵州地方山羊品种间具有较近的亲缘关系,遗传变异较小,具有较高的遗传稳定性。

关 键 词:贵州  山羊  品种  RAPD  遗传多样性  亲缘关系  遗传距离  地方品种
文章编号:0254-5853(2004)02-0141-06

RAPD Analysis on Guizhou Native Goat Breeds
CHEN Xiang ,LIAO Zheng lu ,LI Guo hong ,Zhang Yun ,JIAN Cheng song ,WEI Hong ,LI Hong.RAPD Analysis on Guizhou Native Goat Breeds[J].Zoological Research,2004,25(2):141-146.
Authors:CHEN Xiang  LIAO Zheng lu  LI Guo hong  Zhang Yun  JIAN Cheng song  WEI Hong    LI Hong
Institution:1.Guizhou University,Guiyang 550025,China 2.Livestock Bureau,Guiyang 550001,China 3.Guizhou Normal University,Guiyang 550001,China 4.Third Military Medicinal University,Chongqing 400038,China 5.Shuicheng County,Shuicheng,Guizhou 553000,China
Abstract:We used 180 primers in RAPD analysis of Guizhou native goats (the small xiang goat,Guizhou white goat,Guizhou black goat and Qianbei pockmarked goat),Nanjiang brown goats and Boer goats.Of the primers,27 amplified polymorphic patterns,including 281 bands.Of the bands,115 were polymorphic and their frequency was 40 92% on average (range 20%-80%).Each of the 27 primers had 10 41 bands on average (range 4-16),and the fragment length of products was 210-2?800?bp.Genetic distance index appeared the lowest between the Guizhou black goat and Guizhou white goat (0 0605),and the highest between the Boer goat and the other goats (0 1059-0 1488).NJ tree indicated that the Guizhou white goat was closely related to the Guizhou black goat,and the next was the Qianbei pockmarked goat;the small xiang goat was farther from the other Guizhou native goats than Nanjiang brown goat from.The results analysis suggest that the small xiang goat is a independent breed on genetics,and Guizhou native goat breeds have the close genetic relationship,low genetic variability and high genetic stability.
Keywords:Guizhou native goat  RAPD  Genetic diversity  Relative relationship
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