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微卫星标记对牙鲆有丝分裂雌核发育家系的亲子鉴定
引用本文:季旭,孙效文,杨立更,李雅娟,刘海金.微卫星标记对牙鲆有丝分裂雌核发育家系的亲子鉴定[J].动物学研究,2008,29(1):25-30.
作者姓名:季旭  孙效文  杨立更  李雅娟  刘海金
作者单位:1. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070;大连水产学院生命科学与技术学院,辽宁,大连,116023
2. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070
3. 中国水产科学研究院北戴河中心实验站,河北,秦皇岛,066100
4. 大连水产学院生命科学与技术学院,辽宁,大连,116023
5. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070;中国水产科学研究院,北京,100039
基金项目:国家科技支撑计划(2006BAD01A1207),跨越计划(2006跨18)
摘    要:利用18个微卫星标记,对6个家系的26尾有丝分裂雌核发育牙鲆进行亲子鉴定,PCR扩增产物经8%非变性聚丙烯酰胺凝胶电泳检测,结果表明:1个座位在母本中表现为相同的基因型,视为单态座位,其他17个座位为多态;多态座位在亲子鉴定中的累计排除概率和累计个体识别概率分别为0.9985、0.9999;根据被测个体在17个微卫星座位的基因型,最后确认26尾子代的母本,其中7尾子代在某些座位表现出与其母本不完全匹配的基因型。利用微卫星标记可确定雌核发育后代的亲子关系,从而构建牙鲆雌核发育家系系谱,对牙鲆雌核发育的深入研究具有重要意义。

关 键 词:牙鲆  雌核发育  微卫星标记  亲子鉴定
文章编号:0254-5853-(2008)01-0025-06
收稿时间:2007-10-23
修稿时间:2007-12-27

Paternity Testing of Mitogynogenetic Japanese Flounder (Paralichthys olivaceus) Using Microsatellite Loci
JI Xu,SUN Xiao-wen,YANG Li-geng,LI Ya-juan,LIU Hai-jin.Paternity Testing of Mitogynogenetic Japanese Flounder (Paralichthys olivaceus) Using Microsatellite Loci[J].Zoological Research,2008,29(1):25-30.
Authors:JI Xu  SUN Xiao-wen  YANG Li-geng  LI Ya-juan  LIU Hai-jin
Institution:1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;
2. Life Science and Technology Institute, Dalian Fisheries University, Dalian 116023, China;
3. Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China;
4. Chinese Academy of Fisheries Science, Beijing 100039, China
Abstract:Eighteen microsatellite markers were analysed with a paternity testing application using 26 mitogynogenetic Japanese flounder (Paralichthys olivaceus) progeny and their six assumed female-parents. The amplified products of PCR were analyzed by 8% polyacrylamide gel electrophoresis. Electrophoresis patterns showed that only one pair of primers had a monomorphic pattern, and the others were polymorphic. Cumulate exclusion probability of polymorphic loci was 0.9985 and cumulate power of discrimination was 0.9999. An effective method was applied to track the gynogenetic maternal parents of 26 progeny. The genotypes of six progeny were not completely identical to their mothers on some loci. The construction of pedigree was perfected by looking at microsatellite loci from paternity tests which are essential to the deeper analysis of gynogenetic Japanese flounder.
Keywords:Japanese flounder (Paralichthys olivaceus)  Gynogenesis  Microsatellite marker  Paternity testing
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