三疣梭子蟹Profilin基因全长cDNA的克隆与表达分析

三疣梭子蟹（Portunus trituberculatus）是我国沿海重要养殖品种之一，近年来养殖病害呈逐年上升趋势，制约了三疣梭子蟹养殖产业的健康可持续发展。克隆三疣梭子蟹免疫相关基因，研究免疫基因的功能和作用机制，可为三疣梭子蟹养殖病害的防治奠定基础。本研究从三疣梭子蟹血细胞全长cDNA文库中克隆了742 bp 的profilin基因全长cDNA。Profilin全长cDNA中开放阅读框长375 bp，编码125 aa。推导的三疣梭子蟹profilin理论等电点pI 5.87，氨基酸序列与冈比亚按蚊（Anopheles gambiae）profilin同源性最高，序列一致性为42.9%。荧光定量RT-PCR分析结果显示在正常的三疣梭子蟹机体中，血细胞profilin表达水平最高，其次为肝胰脏；在致病菌副溶血弧菌（Vibrio parahaemolyticus）诱导后，血细胞中profilin表达量显著上升（P<0.01），表明profilin可能参与了三疣梭子蟹的免疫防御反应，是一个免疫相关因子。

cDNA Cloning, Characterization and mRNA Expression of a Profilin from the Swimming Crab Portunus trituberculatus

We isolated and characterized the profilin full-length cDNA from hemocytes of swimming crab Portunus trituberculatus. The profilin cDNA consists of 742 bp and the 375 bp open reading frame encodes a polypeptide of 125 amino acids, having a predicted isoelectric point of 5.87. The deduced amino acid sequence shows 42.9% amino acid sequence identity to the profilin of mosquito Anopheles gambiae. The profilin mRNA was highly expressed in hemocytes and moderately in hepatopancreas of normal crab. The higher exp...