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白yuan胃肠道内分泌细胞的免疫组织化学定位
引用本文:刘斌,曾少举,林永达,张春雷,李福来.白yuan胃肠道内分泌细胞的免疫组织化学定位[J].动物学研究,2004,25(5):442-446.
作者姓名:刘斌  曾少举  林永达  张春雷  李福来
作者单位:1.北京师范大学 生命科学学院,北京 100875 2.北京动物园,北京 100044
摘    要:为了解白yuan的消化生理过程,利用ABC免疫组织化学方法,对白yuan胃肠道5种内分泌细胞:5-羟色胺(5-HT)、脑啡肽、P物质、胃动素和抑胃素的分布进行了研究。结果表明:5-HT细胞主要分布于十二指肠及其以下部位,尤其是盲肠和直肠;而腺胃、肌胃和幽门中均未发现该类细胞。脑啡肽和P物质细胞数量明显较5-HT少,它们稀疏分布于十二指肠及其以下部位,无显著的分布积聚区。抑胃素和胃动素在整个胃肠道中均无分布。分析表明白yuan胃肠道5种内分泌细胞的分布与其他鸟类相似,但与其他脊椎动物明显不同。

关 键 词:白yuan  胃肠道  内分泌细胞  5-羟色胺  脑啡肽  P物质

Location of Endocrine Cells in the Gastrointestinal Tract of White Ibis
Institution:1.College of Life Sciences,Beijing Normal University,Beijing 100875,China 2.Beijing Zoo,Beijing 100044,China
Abstract:For further understanding the digestive physiology of white Ibis,by employment of ABC (streptavidin-peroxidase) immunohistochemical method we studied the distribution of endocrine cells containing 5-hydroxytryptamine (5-HT),met-enkephalin (ENK),substance P (SP),motin and gastric inhibiting polypeptide (GIP) in the gastrointestinal tract of white Ibis (Threskiornis melanocephalus).The results were as follows:5-HT immunoreactive cells were located throughout the gastrointestinal tract with the highest density in the cecum.However,these immunoreactive cells were nearly absent from the stomach.The cells containing ENK and SP were located in the intestine,but they were far sparser than 5-HT cells.Either ENK or SP cells were not congregated throughout the gastrointestinal tract.The cells immunoreactive to motin and GIP were not observed in the gastrointestinal tract of white Ibis.The analysis suggests that the distribution above endocrine cells in the gastrointestinal tract of white Ibis resembles in other bird species,but differs in other vertebrates.
Keywords:White Ibis  Gastrointestinal tract  Endocrine cell  5-hydroxytryptamine  Met-enkephalin  Substance P
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