非人灵长类的体外受精和胚胎移植

非人灵长类的体外受精和胚胎移植是了解人类生殖机制,如卵的成熟调控,受精卵的成熟与分化,胚胎着床,控制某些遗传疾病以及保护珍稀灵长类和提高实验灵长类质量的有效途径。本文从非人灵长类卵的获取(包括超数排卵,非激素刺激动物取卵),精子处理(精液采集,冻存和精子获能),体外受精和胚胎移植、胚胎的冻存等方面介绍了有关研究概况和发展动态。

IN VITRO FERTILIZATION AND EMBRYO TRANSFER IN NON-HUMAN PRIMATES

In vitro fertilization and embryotransfer (IVF-ET) is an useful way to study mechanism of reproduction, for instance, the regulation of oocytematuration, the role of maturation and development of fertilized eggs , embryo implantation as well as a genetic disease model for human. The technology is also helpful for conservation endangered species in nonhuman primates and enhance the quality for laboratory primates. This paper was summarized the progress of IVF- ET in nonhuman primates including oocytes got from hyperstimulation and unstimulated animals, semen collection, semen cryopreservation, fertilization in vitro, embryo transfer and embryo cryopreservation.