扬子鳄4个Sox基因保守区的克隆及序列分析

参考人SRY基因HMG－box的保守区序列，设计一对简并引物，用PCR扩增了扬子鳄Sox基因的HMG－box，并对PCR产物进行了亚克隆和测序。结果在雌雄个体中均筛选到4个不同的Sox基因，无性别差异。其序列与人相应的SOX基因保守区编码序列的相似性分别为91％、96％、100％、96％，分别命名为AS－Sox1,ASSox2,ASSox11,ASSox22。与其他动物相关的Sox/SOX基因的聚类分析结果表明，扬子鳄Sox基因编码的氨基酸序列与进化位置各异的其他动物的Sox/SOX基因编码的氨基酸序列存在高度的同源性，显示出Sox基因在系统进化上的高度保守性。

Amplification and Sequencing of Sox Gene HMG-box in Alligator sinensis

bp fragments of Sox gene HMG box in Alligator sinensis were amplified by PCR,and then sub cloned and sequenced.Four different Sox genes were thus identified in both male and female.The encoded amino acid sequences exhibited 91%,96%,100%,and 96% of sequence homology with those reported for human SOX gene HMG boxes,respectively.According to nomination system of human SOX genes,the newly identified Alligator sinensis Sox genes were named as ASSox 1, ASSox 2, ASSox 11, ASSox 22,respectively.There was no sexual difference in the four fragments.The amino acid sequences coded by Sox genes from amphibian,reptile,bird,and human showed a high degree of sequence homology within the respective four clusters,as revealed in phylogenetic tree constructed.The results indicated that Sox genes were highly conservative in phylogeney.