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树鼩干扰素家族的基本构成及分子特征分析
作者姓名:Li ML  Tian WW  Gao YD  Guo Y  Huang JF  Zhang HT
作者单位:1. 中国科学院昆明动物研究所 动物模型与人类疾病机理重点实验室, 云南 昆明 650223;
2. 中国科学院研究生院, 北京 100049;
3. 西南大学 生物技术学院, 重庆 北碚 400715;
4. 中国科学院昆明生物多样性大型仪器区域中心, 云南 昆明 650223;
5. 中国科学院昆明动物研究所 遗传资源与进化国家重点实验室, 云南 昆明 650223
基金项目:国家重点基础研究发展计划(“973”计划)分课题(2007CB512807); 中国科学院知识创新工程重要方向项目(KSCX2-EW-R-12); 中国科学院基础前沿研究专项项目(KSCX2-EW-J-23); 中科院昆明动物研究所“动物模型与人类疾病机理重点实验室”开放课题
摘    要:干扰素(IFN)是在"危险信号"刺激下,由细胞分泌的具有抗病毒、抗肿瘤、抑制细胞增殖和免疫调节等多重作用的糖蛋白家族,在机体免疫系统中具有重要地位。树鼩作为多种人类疾病研究模型的前景已受到广泛关注,但对其IFN家族的研究尚属空白。该研究在现有的树鼩全基因组数据基础上,应用大片段核酸序列比对、基因预测等方法,对树鼩IFN家族的基本构成和分子特征进行预测和分析。结果显示,树鼩具有I型IFN:α(5个亚型)、β、ω、κ、ε、δ;II型IFN-γ;III型IFN:IFN-λ1、λ2/3,所编码的氨基酸序列及蛋白空间结构与其它哺乳动物具有较高的相似性,但在半胱氨酸位置和N糖基化个数上具有部分差异。该研究以全基因组数据对树鼩IFN家族信息进行系统挖掘和分析,为树鼩IFN的基因克隆以及其在感染免疫学中的作用和机理研究奠定了基础。

关 键 词:树鼩  干扰素  大片段核酸序列比对  基因预测
收稿时间:2011-10-24
修稿时间:2012-01-10

Genome-wide prediction of interferon family members of tree shrew and their molecular characteristics analysis
Li ML,Tian WW,Gao YD,Guo Y,Huang JF,Zhang HT.Genome-wide prediction of interferon family members of tree shrew and their molecular characteristics analysis[J].Zoological Research,2012,33(1):67-74.
Authors:Li Ming-Li  Tian Wei-Wei  Gao Yue-Dong  Guo Yan  Huang Jing-Fei  Zhang Hua-Tang
Institution:Chinese Academy of Sciences, Kunming, China.
Abstract:Interferons (IFNs) represent proteins with antiviral activities that are secreted from cells in response to a variety of stimuli. In addition to antiviral, antibacterial and anti-parasitic host-defense functions they are now also recognized as crucial regulators of cell proliferation, differentiation, survival and death as well as activators of specialized cell functions particularly in the immune system and play important roles in infectious and inflammatory diseases, autoimmunity and cancer. Tree shrews (Tupaia belangeri) were found to be susceptible to several human viruses and therefore are widely regarded as good models for analyzing mechanism of human diseases. In this report, we have forecasted the interferon family members of tree shrew from its genome mainly using the methods like Blast (whole genome shotgun sequence) and gene prediction. Our data show that tree shrew interferon system includes: type I IFN: α (five subtypes), β, ω, κ, ε, δ; type II IFN: γ; type III IFN: λ1, λ2/3. Furthermore, the predicted structures of α and λ have similar character with those of other mammals. However, there are some differences in cysteine position and N-glycosylation numbers between human and Tree shrew IFNs. These results provide fundamental basis for further molecular cloning and function analysis of tree shrew IFNs in future.
Keywords:Tree shrews  Interferon  Whole genome sequence alignment  Gene prediction
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