烟实夜蛾类滞育激素基因的分子克隆

根据烟实夜蛾（Helicovperpa assulta)的性信息素合成激活肽基因序列设计引物，以烟实夜蛾基因组DNA为模板进行PCR扩增，得到665bp的特异性片段。该片段经过同源比较和活性测定，证实烟实夜蛾的基因组中存在类滞育激素基因。烟实夜蛾的类滞育激素是一个由24个氨基酸组成的神经肽，在第4和第5个氨基酸之间，插入了一个482bp的内含子。进一步的分析表明，该神经肽在蛹期的食道下神经节中表达。

Molecular Cloning of Diapause Hormone Analog in Helicoverpa assulta

Diapause hormone (DH) is a neurohormone which is secreted from suboesophageal ganglion and responsi-ble for induction of enbryonic diapause in Bombyx mori. Here, using bioassay, we present evidence that Has-SGNP Ⅰ ,which clearly exhibited diapause egg inducing activity as does Bom-DH, is likely to be DH. We reported the molecularcharacterization of the Helicoverpa assulta diapause hormone analog by polymerase chain reaction. A 665 bp fragment con-taining the entire DH-like gene was isolated and characterized. DH-like gene comprised two exons interspersed by a singleintron, and the consensus sequence for splicing junction is identified at the exon/intron boundary. The result shows thatDH-like gene is localized in the genome of Helicoverpa assulta.