基于线粒体12S和16S rRNA基因部分序列探讨蚱科12种的系统发育关系

用ABI377自动测序仪测定了蚱科5属11个种的12s和16S rRNA基因部分序列，并从GenBank获得1属1种的同源序列；用Clustal X1．81比较其同源性，用Mega2．1计算序列变异性和遗传距离。在获得的736bp序列中，A T含量为71．2％～77．5％，平均为73．9％；G C含量为22．5％～28．8％，平均为26．1％。经Clustal X1．81软件比对，共得到755个位点，其中简约信息位点185个。以Cylindraustralia kochii为外群，构建NJ、MP和ML分子系统树，结果表明：(1)蚱属并非一个单系群，而是一个并系群；(2)环江柯蚱Coptltettix huanjiangensis和贡山柯蚱C.gongshanensis为同一个种，即贡山柯蚱，而环江柯蚱是贡山柯蚱的同物异名。

Phylogenetic Relationships among 12 Species of Tetrigidae (Orthoptera:Tetrigoidea) Based on Partial Sequences of 12S and 16S Ribosomal RNA

Mitochondrial 12S and 16S ribosomal RNA genes sequences were sequenced using dye-labeled terminator on an ABI 377 automated sequencer in 11 individuals and 1 species' sequences were gained from GenBank,representing 6 genera of family Tetrigidae.The collated sequences were aligned using Clustal X version 1.81 and then,the sequence variability and heredity distances based on Kimura 2-parameter model were calculated using Mega 2.1.In obtained sequences (736 bp),the average A T content is 73.9%,ranging from 71.2% to 77.5%;the overall G C content is 26.1%,ranging from 22.5% to 28.8%.Based on alignment of the combined sequences,185 parsimony-informative sites were revealed in 755 available base pairs.Phylogenetic trees were reconstructed using NJ,MP and ML methods with Cylindraustralia kochii as outgroup.The results indicated that the monophyletic nature of Tetrix is questioned and suggest that T.tubercarina may be given tribal rank.Our results also show that Coptltettix huanjiangensis and C.gongshanensis are the same species,i.e.Coptltettix gongshanensis Zheng,and C.huanjiangensis is the synonyms of C.gongshanensis.