兔骨形态发生蛋白15 cDNA部分序列的克隆及其在卵母细胞中的表达(英文）

采用RT-PCR法从新西兰大白兔卵巢中克隆出BMP15基因部分cDNA片段，经blast分析后，发现其与猪、牛、绵羊、山羊、人和小鼠的同源性达到了83%—90%。同时，利用实时荧光定量RT-PCR方法检测了BMP15 mRNA在卵母细胞体外成熟培养过程中（IVM）的表达变化情况，结果表明：在兔卵母细胞体外成熟过程中，该基因在未成熟卵母细胞中表达水平较低，在培养16 h其表达丰度达到峰值，并与卵丘扩展时间相一致，随后下降，推测BMP15可能在兔卵丘细胞扩展中发挥重要作用。

Cloning of Rabbit Bone Morphogenetic Protein 15 and Its Expression During in vitro Maturation of Rabbit Oocytes

Partial cDNA sequence of rabbit BMP15 was cloned by RT-PCR from rabbit ovaries, showing a similarity of 83%−90% with the BMP15 nucleotide sequences in humans, mice, ovine, sheep, cows and pigs. The expression of BMP15 in rabbit cumulus-oocyte complexs during oocytes in vitro maturation (IVM) was measured by fluorescent quantitative RT-PCR method. BMP15 was expressed at low levels in immature oocytes and increased to the highest level at 16 h of IVM, which coincides with the time of cumulus cell expansion, then declined slowly under IVM cultivation. The expression pattern of BMP15 suggested that it might be important in cumulus expansion in rabbits.