林氏果蝇种群间mtDNA的比较研究

运用１０种限制性内切酶分析了林氏果蝇来自不同地区的２２个单雌系的线粒体ＤＮＡ限制性片段长度多态性。采自台湾省的ＴＡＷ３１４６．１与大多数采自广东的品系相同，而广东省品中则存在一定度的分化，ＤＨＳ３０７、３１５和ＮＫＳ９２３４仅在ＨｉｎｄⅢ的酶切位点上与其余单雌系不同；ＴＡＷ３１４６．１与来自缅甸中部的ＭＭＹ３０７，ＭＤＭＹ３２６之间的遗传距离为０．００２７４；ＭＭＹ３０７与ＭＭＹ３２６之间无差异。

STUDIES ON MITOCHONDRIAL DNA COMPARISONS OF Drosophila lini WITHIN DIFFERENT POPULATIONS

Mitochondrial DNA (mtDNA) variation was investigated among 22 isofemale lines from different populations of Drosophila lini with 10 restriction endonucleases. The isofemale lines were collected originally from Taiwan, Guangdong in China and Yangon (Rangoon), Pyinoolwin (Maymyo) in Myanmar. Comparison of the cleavage sites allowed us to build a phylogenetic tree based on genetic distances of isofemale strains. The results show as follows: 1) The mtDNA nucleomorph is the same in isofemale lines from Taiwan (TAW 3146.1) and Guangdong except DHS 307, 315 and NKS 9243 from Guangdong. 2) The difference is not found between MMY 307 and 326. 3) The strains from Myanmar might have diverged. The genetic distance between TAW 3146.1 and RGN 3, RGN 206 is 0.00534 and 0.00553, between MMY 307, 326 and RGN 3, RGN 206 is 0.00835 and 0.00868. 4) The genetic distances between MMY 307, 326 and TAW 3146.1 is very close (0.00274). As the same as between DHS 307, 315, NKS 9243 and TAW 3146.1