| 家鸽Caveolin-1基因全长cDNA的克隆、序列和组织表达分析 |
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| 作者姓名： | Zhang Y Yu JF Yang L Wang XG Gu ZL |
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| 作者单位： | 1. 苏州大学,基础医学与生物科学学院,江苏,苏州,215123;常熟理工学院,生物科学与工程系,江苏,常熟,215500
2. 常熟理工学院,生物科学与工程系,江苏,常熟,215500 |
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| 基金项目： | 江苏省“六大人才高峰”第六批高层次人才项目; 江苏省自然科学基金(BK2005026) |
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| 摘 要： | 窖蛋白(Caveolin)是一类构成细胞膜上胞膜窖主要结构的标志蛋白,由Caveolin基因家族编码而成。Caveolin-1基因是Caveolin基因家族的成员之一。该实验采用RT-PCR与RACE(rapid amplification of cDNA ends)技术成功地克隆了家鸽Caveolin-1基因的全长cDNA。该cDNA全长2605bp,包含537bp的完整编码区,编码178个氨基酸;分析发现家鸽Caveolin-1基因编码区与牛、家犬、鸡、褐家鼠等核苷酸同源性为80.1%～93.4%,氨基酸同源性高达85.4%～97.2%;半定量RT-PCR分析表明该基因在家鸽各种组织广泛表达,脂肪中表达量最高,各种肌肉中表达量次之,肝脏中表达量最低。此结果说明家鸽Caveolin-1基因可能与脂肪、肌肉中的某些代谢途径有关。
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| 关 键 词： | 家鸽 窖蛋白基因 RACE 组织表达分析 |
Cloning and characterization of Caveolin-1 gene in pigeon, Columba livia domestica |
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| Zhang Y,Yu JF,Yang L,Wang XG,Gu ZL.Cloning and characterization of Caveolin-1 gene in pigeon, Columba livia domestica[J].Zoological Research,2010,31(5):476-482. |
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| Authors: | Zhang Ying Yu Jian-Feng Yang Li Wang Xing-Guo Gu Zhi-Liang |
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| Institution: | ZHANG Ying 1,2,YU Jian-Feng 2,YANG Li 2,WANG Xing-Guo 1,GU Zhi-Liang 2,(1. College of Preclinical Medicine and Biological Science,Soochow University,Suzhou Jiangsu 215123,China,2. Department of Life Science and Technology,Changshu Institute of Technology,Changshu Jiangsu 215500,China) |
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| Abstract: | Caveolins, a class of principal proteins forming the structure of caveolae in plasmalemma, were encoded by caveolins gene family. Caveolin-1 gene is a member of caveolins gene family. In the present study, a full-length of 2605 bp caveolin-1 cDNA sequence in Columba livia domestica, which included a 537 bp complete ORF encoding a 178 amino acids long putative peptide, were obtained by using RT-PCR and RACE technique. The Columba livia domestica caveolin-1 CDS shared 80.1% ? 93.4% homology with Bos taurus, C... |
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| Keywords: | Columba livia domestica Caveolin gene Rapid amplification of cDNA ends Tissue expressional pattern |
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