Immunocompetence of macrophages in rats exposed to Candida albicans infection and stress |
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Authors: | Rodriguez-Galán Maria Cecilia Sotomayor Claudia Costamagna Maria Eugenia Cabanillas Ana Maria Rentería Beatriz Salido Masini-Repiso Ana Maria Correa Silvia |
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Institution: | Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Pabellón Argentina 5000, Córdoba, Argentina. |
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Abstract: | The integration of innate andadaptive immune responses is required for efficient control ofCandida albicans. The present work aimed to assess, at thelocal site of the infection, the immunocompetence of macrophages inrats infected intraperitoneally with C. albicans and exposedsimultaneously to stress during 3 days (CaS group). We studied the1) ability to remove and kill C. albicans,2) tumor necrosis factor- (TNF-) release,3) balance of the inducible enzymes NO synthase (iNOS) andarginase, and 4) expression of interleukin (IL)-1 andIL-1 receptor antagonist (ra) mRNA. Compared with only infected animals(Ca group), the number of colony-forming units was significantly higherin CaS rats (P < 0.01), and the macrophagecandidicidal activity was ~2.5-fold lower (P < 0.01). Release of TNF- was diminished in both unstimulated andheat-killed C. albicans restimulated macrophages of the CaSgroup (Ca vs. CaS, P < 0.03 and P < 0.05, respectively). In Ca- and CaS-group rats, the rates for both thearginase activity and the NO synthesis were significantly enhanced.However, the stress exposure downregulated the activity of both enzymes(CaS vs. Ca, P < 0.05). After in vitro restimulation,the IL-1ra/IL-1 ratio was significantly diminished in CaS-group rats(P < 0.05). Our results indicate that a correlationexists between early impairment of macrophage function and stress exposure. |
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