Expression profile of rrbp1 genes during embryonic development and in adult tissues of Xenopus laevis
Institution:
1. Key Laboratory for Regenerative Medicine of Ministry of Education and Department of Developmental & Regenerative Biology, Ji Nan University, Guangzhou 510632, China;2. School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, People''s Republic of China;3. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China;4. Department of Dermatology and Institute of Hair and Cosmetic Medicine, Yonsei University Wonju College of Medicine, Wonju, Gangwon 220-701, Korea;5. Department of Physiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 220-701, Korea;6. Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 220-701, Korea;7. Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 220-701, Korea;1. Key Laboratory of Regenerative Medicine (JNU-CUHK), Ministry of Education and Department of Developmental & Regenerative Biology, Ji Nan University, Guangzhou 510632, China;2. Department of Physiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 220-701, South Korea;3. School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong, China;4. Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 220-701, South Korea;1. Department of Bioengineering, University of Illinois at Chicago, USA;2. Department of Biomedical Engineering, Yonsei University, Wonju, Korea;3. Center for Nano-Bio Measurement, Korea Research Institute of Standards and Science, Daejeon, 34113, Korea
Abstract:
Recent studies suggest that ribosome-binding protein 1 (RRBP1) is involved in multiple diseases such as tumorigenesis and cardiomyopathies. However, its function during embryonic development remains largely unknown. We searched Xenopus laevis database with human RRBP1 protein sequence and identified two cDNA sequences encoding Xenopus orthologs of RRBP1 including rrbp1a (NM_001089623) and rrbp1b (NM_001092468). Both genes were firstly detected at blastula stage 8 with weak signals in animal hemisphere by whole mount in situ hybridization. Evident expression of rrbp1 was mainly detected in cement gland and notochord at neurula and tailbud stages. Heart expression of rrbp1 was detected at stage 36. RT-PCR results indicated that very weak expression of rrbp1a was firstly detected in oocytes, followed by increasing expression until stage 39. Differently, very weak expression of rrbp1b was firstly observed at stage 2, and then maintained at a lower level to stage 17 followed by an intense expression from stages 19–39. Moreover, both expression profiles were also different in adult tissues. This study reports Xenopus rrbp1 expression during early embryonic development and in adult tissues. Our study will facilitate the functional analysis of Rrbp1 family during embryonic development.
