Characterization of Sgo1 expression in developing and adult mouse |
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| Institution: | 1. Cardiovascular Genetics, Department of Pediatrics, CHU Sainte-Justine, Montreal, QC, Canada;2. Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK;3. Université de Montréal, Montreal, QC, Canada;4. McGill University, Department of Anatomy and Cell Biology, Montreal, QC, Canada;5. Department of Medicine, Montreal Heart Institute and Université de Montréal, Montreal, QC, Canada;6. Department of Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada;7. Institute of Pharmacology, West German Heart and Vascular Center, Faculty of Medicine, University Duisburg-Essen, Essen, Germany;1. Université de Montréal, Département de biochimie, Montréal, Québec H3C 3J7, Canada;2. Division of Cancer Biology and Genetics, Queen’s University, Cancer Research Institute, Kingston, Ontario K7L 3N6, Canada;3. Department of Biochemistry, Queens University, Kingston, Ontario, Canada;1. Department of Medicine, The University of Oklahoma Health Sciences Center, United States;2. Department of Physiology, The University of Oklahoma Health Sciences Center, United States;3. Department of Ophthalmology, The University of Oklahoma Health Sciences Center, United States;4. Harold Hamm Diabetes Center, The University of Oklahoma Health Sciences Center, United States;5. Dean A. McGee Eye Institute, The University of Oklahoma Health Sciences Center, United States;1. Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA;2. Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA;3. Bioinformatics and Molecular Genetics, Faculty of Biology, Albert-Ludwigs-University Freiburg, Freiburg, Germany;1. Department of Radiation Oncology, Gustave Roussy Cancer Campus, Villejuif, France;2. Department of Radiology and Medical Physics, Medical School, University of Cordoba, Spain;3. Institut Curie, Paris, France;4. PSL Research University, Paris, France;5. Inserm, U900, Paris, France;6. Mines Paris Tech, Fontainebleau, France;7. CNRS, UMR144, Paris, France;8. Department of Radiation Oncology, IUCT Oncopole, Toulouse, France;9. Service de Biostatistique et d’Epidemiologie, Gustave Roussy, Villejuif, France;10. INSERM U1018, CESP, Université Paris-Sud, Université Paris-Saclay, Villejuif, France;11. Department of Medical Oncology, Institut Curie, Paris, France;12. Department of Radiation Oncology, Reina Sofia University Hospital, Cordoba, Spain;1. Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera, No. 239, Fraccionamiento “La Escalera”, Ticomán, C.P. 07320 México DF, Mexico;2. ID21, European Synchrotron Radiation Facility, 6 Rue Jules Horowitz, 38000 Grenoble, France;3. Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo envolvente Pronaf y Estocolmo s/n, 32310 Cd. Juárez, Mexico |
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| Abstract: | SGO1 has been characterized in its function in correct cell division and its role in centrosome cohesion in the nucleus. However, its organ-specific maturation-related expression pattern in vivo remains largely uncharacterized. Here, we show clear SGO1 expression in post-developmental neuronal cells and cytoplasmic localisation in nucleated cells with a transgenic mice model and immunohistochemistry of wild type mice. We demonstrate extranuclear expression of Sgo1 in the developing heart and gut, which have been shown to be dysregulated in humans with homozygous SGO1 mutation. Additionally, we show Sgo1 expression in select population of retinal cells in developing and post-developmental retina. Our expression analysis strongly suggests that the function of SGO1 goes beyond its well characterized role in cell division. |
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| Keywords: | SGO1 Cohesin Gut development Heart development Eye development |
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