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Quantifying difference in gene expression profile between bovine blastocysts derived by in vitro fertilization and somatic cell nuclear transfer |
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| Institution: | 1. Development and Differentiation Research Center, KRIBB, 111 Gwahang-ro, Yuseong-gu, Daejeon 305-806, South Korea;2. Department of Functional Genomics, University of Science and Technology (UST), 113 Gwahang-ro, Yuseong-gu, 305-333 Daejeon, South Korea;3. Division of Medicine Research, Korea Institute of Oriental Medicine, 483 Expo-ro, Yuseong-gu, Daejon 305-811, South Korea;1. Division of Genetics and Tree Improvement, Institute of Forest Biodiversity (ICFRE), Hyderabad, India;2. Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, India;3. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India;1. Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan;2. Division of Genomics Research, Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan;3. Department of Neurology, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan;1. Development and Differentiation Research Center, Aging Convergence Research Center (ACRC), Korea Research Institute of Bioscience Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, South Korea;2. Department of Functional Genomics, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, South Korea;1. School of Pharmacy, Chung-Ang University, Seoul, 06974, South Korea;2. Department of Life Science, Chung-Ang University, Seoul, 06974, South Korea;3. Department of Obstetrics and Gynecology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, South Korea;4. Department of Biochemistry, Chosun University School of Medicine, 309 Pilmoondaero, Gwangju, South Korea |
| Abstract: | Epigenetic reprogramming intensely occurs in somatic-cell nuclear transfer (SCNT) embryos, which highlights the importance of proper expressions of reprogramming-related genes in SCNT embryos. We here assessed gene expression profiles (GEPs) difference between bovine blastocyst groups derived by in-vitro fertilization (IVF) or SCNT; in SCNT, cumulus cells and ear skin fibroblasts were used for cSCNT and fSCNT blastocysts, respectively. We obtained GEPs of 15 reprogramming-related genes in single blastocysts using multiplex PCR and found a broad range of variations in their GEPs. Weighted root-mean-square deviation (wRMSD) analysis, which calculates the deviation of SCNT blastocysts' GEPs from IVF blastocysts' mean GEP, found a significant difference between IVF and fSCNT and between cSCNT and fSCNT blastocysts (p < 0.001) but not between IVF and cSCNT. Since the fibroblasts' GEP was more distant from the IVF blastocysts' than the cumulus cells', it might partly explain the less similarity of fSCNT blastocysts' GEPs to the IVF's mean GEP. Our wRMSD method succeeds in expressing in figures how different two comparable embryo groups of different derivations are in GEP, which would be useful to select a better embryo derivation protocol among the candidates prior to field applications. |
| Keywords: | Multiplex PCR Embryo Reprogramming wRMSD SCNT"} {"#name":"keyword" "$":{"id":"kwrd0035"} "$$":[{"#name":"text" "_":"somatic cell nuclear transfer cSCNT and fSCNT"} {"#name":"keyword" "$":{"id":"kwrd0045"} "$$":[{"#name":"text" "_":"SCNT using cumulus cell and fibroblast respectively IVF"} {"#name":"keyword" "$":{"id":"kwrd0055"} "$$":[{"#name":"text" "_":"in vitro fertilization wRMSD"} {"#name":"keyword" "$":{"id":"kwrd0065"} "$$":[{"#name":"text" "_":"weighted root mean square deviation GEP"} {"#name":"keyword" "$":{"id":"kwrd0075"} "$$":[{"#name":"text" "_":"gene expression profile |
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