Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae) |
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| Authors: | Tavares Mara Garcia Carvalho Carlos Roberto Soares Fernanda Aparecida Ferrari Fernandes Anderson |
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| Institution: | 1.Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brazil;2.Departamento de Ciências Biológicas, Universidade do Estado de Mato Grosso, Mato Grosso, MT, Brazil |
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| Abstract: | When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes. |
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| Keywords: | cytogenetic flow cytometry genome size karyotype stingless bee |
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